What particle diameter is needed to give 15 000 plates over a 10-cm HPLC column?
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What particle diameter is needed to give 15 000 plates over a 10-cm HPLC column?
A HPLC column was operated under the following conditions
Column Length – 25.4 cm Column
Diameter = 0.5 mm
Mass stationary phase = 0.49
g Density
Stationary Phase = 1.12 g/mL
Measured Outlet Flow Rate = 1.0 mL/min
Column Temperature = 25 oC.
resolution equation=Rs = Trb-Tra/W
Plate height(H) = length of column (L)/Number of plate on the
column (N)
No. of theoretical N= 16(RS/w)2
Calculate the resolution for components #3 and #4?
Calculate the plate height for column (use...
HPLC
4. (2pts) Make sure you explain your answers. N = 2 000 plates N=7 500 plates etector response A) Which chromatogram represents a separation that is more efficient? Why? B) How does the particle size of the packing material in the column reflect on the separation? Use an approximate size of the particles to illustrate the difference in chromatogram a) and b). del lagog toy 912 M H E lo missib bold a test2 21GE) Trenden anlato ingilis
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help needed with this problem set
Refer to the HPLC chromatograms below for a 5-component mixture of organic acids for the following questions. The specific HPLC parameters for chromatogram A (top) are described in the figure title and the insert. You only need the lecture notes and/or your personal experience to answer those questions. Mobile phase: 30 % MeCN, 70 % TFA, 0.1 M (pH 2.0) low rate: 1.0 mL/min 2Benzok Acid Maleic Aci 1. (a) Which component has the...
A 10-cm-diameter parallel-plate capacitor has a 1.0 mm spacing. The electric field between the plates is increasing at the rate 1.3×10^6 V/ms . a) What is the magnetic field strength 3.2 cm from the axis?
15)A parallel-plate capacitor is constructed of two horizontal 17.2-cm-diameter circular plates. A 1.9 g plastic bead with a charge of -7.2 nC is suspended between the two plates by the force of the electric field between them.
When the particle exits the region between the plates, what
will be the magnitude of its vertical displacement from its entry.
( in mm)
lass Management | Help ectrostatic Charge Begin Date: 1/17/2018 4:01:00 PM-Due Date: 1/25/2018 11: (1000) Problem 2: A particle with charge q = +4e and mass m-76x 10-26 kg is injected horizontally with speed 1.9x106 m/s into the region between two parallel horizontal plates. The plates are 26 cm long and 1.3 cm apart. The particle...