Homework Answers
- The media to be used in the lipid hydrolysis test is tributyrin agar (a mixture of nutrient agar and the triglycerides tributyrin)
- The enzyme produced by the bacteria in the lipid hydrolysis is the exoenzyme lipase
- The products of lipid hydrolysis are
- Glycerol
- Fatty acids
- The process that converts fatty acids to acetyl CoA is called as lipolysis
- The area around or encircling the bacteria is clear in the media which indicates positive test for lipid hydrolysis
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What is the media used for lipid hydrolysis test? What is the enzyme bacteria produce for...
2. What is the enzyme being tested? 3. What is the substrate if the enzyme? 4....
2. What is the enzyme being tested? 3. What is the substrate if the enzyme? 4. For what purpose do bacteria that have the enzyme utilize it? 6. What will occur if the plate is incubated right side up? 7. What will occur if the plate is incubated for more than 24-48 hours? Exercise 7: Selective and Differential Growth Medium and Tests 95 Exercise 7.9 Hydrolytic Exoenzymes: Lipase and Casease LIPASE TEST Lipids are fatty acids used by some bacterium...
Question 5 (1 point) The six test tubes will be in a water bath for 40...
Question 5 (1 point) The six test tubes will be in a water bath for 40 minutes at what temperature range? 30-35 °C 35-40 °C O 30-40 °C 040-45 °C Question 1 (0.5 points) Whole milk contains more fat than skim milk. True False Question 2 (0.5 points) Whole milk, when hydrolyzed, can produce more fatty acids than skim milk can. True False Question 3 (0.5 points) Simple lipids can be hydrolyzed. True False Question 4 (0.5 points) Fatty acids...
40.Fatty acid synthase is an enzyme in the lipid metabolism pathway, that: A. occurs in the...
40.Fatty acid synthase is an enzyme in the lipid metabolism pathway, that: A. occurs in the mitochondrion in animal cells, 2 nor Section A B. utilises NADH as an electron donor C) requires NADPH as a substrate. D. requires acetic acid as a substrate. E. consists of a single multifunctional protein in plants and bacteria. 41.One round of beta oxidation produces 1 molecule of acetyl-CoA from a saturated Acyl-CoA molecule. Which reducing equivalents are made in this process in which...
80.1% Resources Hint Check Answer Animals are not able to convert fatty acids into carbohydrates. The...
80.1% Resources Hint Check Answer Animals are not able to convert fatty acids into carbohydrates. The net synthesis of 1 mol of oxaloacetate from 2 mol of acetyl-CoA by the citric acid cycle does not occur because 1 mol of oxaloacetate is used in the cycle for each one generated. Plants are able to convert 2 mol of acetyl-CoA into 1 mol of oxaloacetate with only two additional enzymes not found in animals. This process is called the glyoxylate cycle,...
• 12. This medium is used to test for hydrolysis: A. starch; B. gelatin; C. lipid....
• 12. This medium is used to test for hydrolysis: A. starch; B. gelatin; C. lipid. • 13. Which organism shows a positive result for this test? Left or right.
Question 1 (1 point) In this lab you will attempt to hydrolyze the triacylglycerols ("fat") found...
Question 1 (1 point) In this lab you will attempt to hydrolyze the triacylglycerols ("fat") found in milk. What are the expected products for this hydrolysis? triacylglcerol and fatty acids fatty acids and glycerol glycerol and water water and triglycerides Question 2 (1 point) In this lab you will attempt to hydrolyze the triacylglycerols ("fat") found in milk. If hydrolysis is successful, what will happen to the pH of the milk solution? pH will increase pH will decrease OpH will...
DNA Hydrolysis Ex. 5.14 . 1. For this test, what is the inoculation method used? What...
DNA Hydrolysis Ex. 5.14 . 1. For this test, what is the inoculation method used? What ingredients does the agar contain? B ritis IN F REFER 2. What enzyme are we testing for in this test and what does it do? 3. Which inoculation tested positive in the DNA Hydrolysis test and which tested negative. How do you know? 1 /8
please help 126) Describe how you could test a solution for the presence of Carbohydrate (starch),...
please help
126) Describe how you could test a solution for the presence of Carbohydrate (starch), or Sugars, Protein, or, Amino Acid, and Lipid or Glycerol and Fatty Acids, What do you have to do in each case (procedure) ? What would a positive reaction look like (appearance)? Procedure Appearance Chemical Carbohydrate (Starch) Sugar Glucose Protein Amino Acids Lipid (Triglycerides) Glycerol and Fatty Acids
3. (2pt) The acetaldehyde produced from the reaction we have been studying in labis poisonous to...
3. (2pt) The acetaldehyde produced from the reaction we have been studying in labis poisonous to eukaryotic cells. However, our cells have an enzyme called acetaldehyde dehydrogenase that can turn this poisonous substance into acetyl-CoA, a molecule that aids in metabolism. Looking at the figure below, what stage of respiration would be most disrupted if acetaldehyde dehydrogenase was inhibited? What molecule in this reaction would be used to observe the rate of the reaction and what frequency would you set...
Are you graduating in December 201 YES/NO 1) Which enzyme is needed for the oxidation of...
Are you graduating in December 201 YES/NO 1) Which enzyme is needed for the oxidation of odd-chain saturated fatty acids that is not needed 1) for even-chain fatty acids? A) methylmalonyl-CoA mutase C) methylmalonyl-CoA racemase B) D) All of the above 2) When red-blood cells are treated extensively with protease most down into small peptides. However, some proteins are very resistant to this treatment. 1f cleavage by protease is the only type of reaction that occurs in this treatment, how...
2. What is the enzyme being tested? 3. What is the substrate if the enzyme? 4. For what purpose do bacteria that have the enzyme utilize it? 6. What will occur if the plate is incubated right side up? 7. What will occur if the plate is incubated for more than 24-48 hours? Exercise 7: Selective and Differential Growth Medium and Tests 95 Exercise 7.9 Hydrolytic Exoenzymes: Lipase and Casease LIPASE TEST Lipids are fatty acids used by some bacterium...
Question 5 (1 point) The six test tubes will be in a water bath for 40 minutes at what temperature range? 30-35 °C 35-40 °C O 30-40 °C 040-45 °C Question 1 (0.5 points) Whole milk contains more fat than skim milk. True False Question 2 (0.5 points) Whole milk, when hydrolyzed, can produce more fatty acids than skim milk can. True False Question 3 (0.5 points) Simple lipids can be hydrolyzed. True False Question 4 (0.5 points) Fatty acids...
40.Fatty acid synthase is an enzyme in the lipid metabolism pathway, that: A. occurs in the mitochondrion in animal cells, 2 nor Section A B. utilises NADH as an electron donor C) requires NADPH as a substrate. D. requires acetic acid as a substrate. E. consists of a single multifunctional protein in plants and bacteria. 41.One round of beta oxidation produces 1 molecule of acetyl-CoA from a saturated Acyl-CoA molecule. Which reducing equivalents are made in this process in which...
80.1% Resources Hint Check Answer Animals are not able to convert fatty acids into carbohydrates. The net synthesis of 1 mol of oxaloacetate from 2 mol of acetyl-CoA by the citric acid cycle does not occur because 1 mol of oxaloacetate is used in the cycle for each one generated. Plants are able to convert 2 mol of acetyl-CoA into 1 mol of oxaloacetate with only two additional enzymes not found in animals. This process is called the glyoxylate cycle,...
• 12. This medium is used to test for hydrolysis: A. starch; B. gelatin; C. lipid. • 13. Which organism shows a positive result for this test? Left or right.
Question 1 (1 point) In this lab you will attempt to hydrolyze the triacylglycerols ("fat") found in milk. What are the expected products for this hydrolysis? triacylglcerol and fatty acids fatty acids and glycerol glycerol and water water and triglycerides Question 2 (1 point) In this lab you will attempt to hydrolyze the triacylglycerols ("fat") found in milk. If hydrolysis is successful, what will happen to the pH of the milk solution? pH will increase pH will decrease OpH will...
DNA Hydrolysis Ex. 5.14 . 1. For this test, what is the inoculation method used? What ingredients does the agar contain? B ritis IN F REFER 2. What enzyme are we testing for in this test and what does it do? 3. Which inoculation tested positive in the DNA Hydrolysis test and which tested negative. How do you know? 1 /8
please help
126) Describe how you could test a solution for the presence of Carbohydrate (starch), or Sugars, Protein, or, Amino Acid, and Lipid or Glycerol and Fatty Acids, What do you have to do in each case (procedure) ? What would a positive reaction look like (appearance)? Procedure Appearance Chemical Carbohydrate (Starch) Sugar Glucose Protein Amino Acids Lipid (Triglycerides) Glycerol and Fatty Acids
3. (2pt) The acetaldehyde produced from the reaction we have been studying in labis poisonous to eukaryotic cells. However, our cells have an enzyme called acetaldehyde dehydrogenase that can turn this poisonous substance into acetyl-CoA, a molecule that aids in metabolism. Looking at the figure below, what stage of respiration would be most disrupted if acetaldehyde dehydrogenase was inhibited? What molecule in this reaction would be used to observe the rate of the reaction and what frequency would you set...
Are you graduating in December 201 YES/NO 1) Which enzyme is needed for the oxidation of odd-chain saturated fatty acids that is not needed 1) for even-chain fatty acids? A) methylmalonyl-CoA mutase C) methylmalonyl-CoA racemase B) D) All of the above 2) When red-blood cells are treated extensively with protease most down into small peptides. However, some proteins are very resistant to this treatment. 1f cleavage by protease is the only type of reaction that occurs in this treatment, how...
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