Question

DNA Hydrolysis Ex. 5.14 . 1. For this test, what is the inoculation method used? What ingredients does the agar contain? B ritis IN F REFER 2. What enzyme are we testing for in this test and what does it do? 3. Which inoculation tested positive in the DNA Hydrolysis test and which tested negative. How do you know? 1 /8

Answer 1

Answero ONA Hydrolysis Ex.5.14 DNA hycholysis is a test used to determine whether a microbe can produce the exoenzyme deoxyribonuclease Conaose) that hydrolyzes DNA and utilize it as a sarce of carbon and energy for growth. DNAose agar, an agar medium is used as the medium during this test. Methyl green (a slain) is used as an indicafor in the growth medium, Methyl green is a cation that binds to negofively charged DNA. * Two types of inoculation methods can be used for DNA hydrolysis. ay Spot inoculofion :- This type of inoculation is done so that all growth is radiated outward from approximately af a single point During this, the colony of organism under test is touched With & loop and is inoculated onto a small area of the DNAase test agar plote. Inoculation is done in the middle of the marked sections so that a thick plaque of growth is formed. The plate is incubated at 37 c for 18 to 24 hours. by Streak inoculation This method is a rapid qualitative isolation method. During this, a heavy inoculum is used and a line of 3 to 4 cm length is drawn from the rim to the center of the DNA ase test agar plate. The plafe is incubated of 37c for 18 to 24 hours.

qum is composed of The ingredients of DNAage ager medium is compos agar, casein and soy peptons which supply necessary, nupa Jodium chloride is added to maintain osmotic equilibrium : is incorporeefed into the medium to defect organisms possess the enzyme DNA ose . Methyl green is used as a indicafor. O DNA hydrolysis test is used to test whether a microbe can produce the expenzyme DNAose. DNAose allows the organisms that produce it to break down DNA into smaller fragment and utilize it as a source of carbon and energy for growth. ③ Methyl green is the indicafor which is a casion that provides opaqueness to a medium in the presence of negofively Charged DNA strands. Whens DNA is broken down, it mo longer binds to methyl green and hence, a clear halo will appear around the areas where the anaose-producing organism has grown. Hence, the test is positive when DNA is hydrolyzed and methyl green is released tuming the medium colorless around the test organism. The test is negofive when the medium remains green os no degradafion of DNA has occured.

PLEASE GIVE POSITIVE RATING IF ANSWER IS SATISFACTORY, THANK YOU!!!