Question

Lab Report: Ex 1-3: Aseptic Techniques and Inoculation Methods Pre-lab Questions: • List two main reasons to use aseptic techniques. • What should you see when your Bunsen burner is properly adjusted? • Which part of the flame you would use to sterilize inoculating instruments • Which hand you should hold your inoculating loop? How do you sterilize your inoculating loop? • Why do we have to hold open tubes at an angle? • Where and how should you hold the test tube caps, when you open the culture tubes? • When opening a petri plate, what you need to do prevent contamination?

Answer 1

1. To prevent contamination from micro organisms

To protect the technician from infection

2. The perfectly adjusted Bunsen burner gives steady blue color flames

3. Use the hottest part of the flame that is the middle part to sterilize the inoculating instruments.

4. The inoculating loop is held in the right hand.

5. Place the handle end of the loop in the hot part of the flame with the loop outside the flame and allow time for heat to be conducted along the wire to the tip of the loop, Heat the inoculating loop until its red hot.

6. It reduces the pressure on the substance allowing any gases to escape harmless into the air without harming anyones face or eye

7. Its always best to hold the cap in your right hand (the hand holding the sterile loop). Curl the little finger of your right hand around the cap to hold it.

8. When ever the lid is removed it should be held over the plate as a shield. Do not place the lid on the bench top. Do not leave the plates uncovered. Always decontaminate the hood before opening the plate and always keep the air flow on while handling a plate