Question

Materials and methods We need to have all our tubes already sterilized, and the petri dish that we are going to use in this experiment. Our dishes must be labelled on the bottom part and the tubes in one side with our initial names, bench number and the number given to the bacteria. Take inoculum tubes, the sterilized tubes, and the plates near to our non-dominant hand. Lift the Bunsen burner: we need to attach the hose of the Bunsen to the closed gas tap. Examine the burner to ensure that the barrel is fully turned into its strand the open the air inlet windows fully. We need to light up the flame to sterilize our materials, the flame will be three tone blue with the inner and second highest cones visible. The inner cone is the hottest flame and we get the best oxidation at this point which is where we hold the nichrome (nickel/chromium) inoculating I loop to sterilize it. The top of the outer cone is the least hot and this is where we pass our air-dried smears for heat fixing. Removing from a liquid culture: we need to sterilize the loop in the top of the flame until it reaches the orange color, after this we need to wait 30 seconds to a minute because if we enter the loop in the sample tube when it is still hot we may kill our sample. after the loop is cold we need to take a sample from one tube, we need to mx it up carefully so they spread in the tube and the tube gets cloudily, we need to be careful, we cannot put the tube upside down because we may spill out the bacteria on us, so, we need to be holding the tube with the sample at an angle of 45 deg. to avoid such as accident. When the loop is sterilized we must hold the sample tube near the flame, pass the mouth of the tube rapidly twice through the top of the flame get the sample by going all the way to the bottom, pass the mouth of the tube through the flame twice again cover it, and finally place the sample on the sterilized tube or plate previously designed for them. After each try we must sterilize the loop again (flame the loop) Transferring the slant: we are going to do all the steps described above, but this time we need to put the loop all the way to the edge of the slant to the very bottom of the slant and move it back and forth along the surface of the slant to take the sample out. Removing the sample from a plate: we do the same thing sterilize the loop take a sample from the plate by rubbing the surface and place it into another medium.

Answer 1

Before starting the experiment, the sterilized petridishes and test tubes are labelled with our initial names, bench number and number given to the bacteria. The nichrome inoculating loop is sterilized by holding the loop in the top of the inner core of the flame, until it reaches the orange colour. When the loop is sterilized, the sample tube is held near the flame, the mouth of the test tube is passed rapidly twice through the top of the flame. After about a minute when the loop is cold, a sample from one tube is taken, mixed up carefully so they spread in the tube. The tube with the sample is held at an angle of 45 degree. The sample is finally placed on the respective sterilized tube or plate. After each try, the loop is sterilized. In order to take the sample out, the loop is put all the way to the edge of the slant to the very bottom and moved back and forth along the surface to take the sample out. To remove the sample from a plate, the loop is sterilized, the sample from the plate is taken by rubbing the surface and placed into another medium.