RECOMBINANT DNA REVIEW 1. What is a nucleic acid probe and what is it used for?...
RECOMBINANT DNA REVIEW
1. What is a nucleic acid probe and what is it used for?
2. How does the DNA ligase enzyme work specificially?
Homework Answers
Answer with explanation is given below :-
1. Nucleic acid probe :- It is a short single stranded molecule of DNA or RNA. The molecule is radioactively or fluorescently labeled and complementary sequence to the interested gene.
Uses of nucleic acid probe :- the nucleic acid probe (Small piece of DNA or RNA) is used to search its complementary sequence in a sample genome . Nucleic acid probes are used to detect mRNA and interphase chromosome in tumour cells .
2. DNA ligase enzyme :- DNA ligase enzyme is a important specific enzyme which is used for joining the DNA strands together by catalyzing the formation of phosphodiester bond . This enzyme also repairs breaks in DNA and it connects okazaki fragments during DNA replication. DNA ligase also use in recombinant DNA technology .
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RECOMBINANT DNA REVIEW
1. What is a nucleic acid probe and what is it used for?...
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2) With reference to production of recombinant DNA answer the following questions: i) what is "plasmid" and how is it isolated from a cell (1 point); ii) what is "insert" (1/2 point); iii) name the enzyme used for cleaving and generating sticky ends on the plasmid and insert (1 point); iv) name the enzyme used for joining sticky ends of plasmid and insert to produce a molecule of recombinant DNA (1 point); v) name the most common microbial host cell...
1. An enzyme used to covalently join DNA segments to form recombinant DNA molecules is called a A. Restriction endonuclease B. Reverse transcriptase C. DNA polymerase D. Helicase E. Ligase 7. The procedure for introducing changes into specific genes is called A. An enhancer trap B. Imprinting C. RT-PCR D. DNA looping E. Gene targeting 2. Plasmids used for in vitro cloning of foreign DNA fragments are called A. Donors B. DNA chips C. Clones D. Vectors E. Conjugants 8....
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