Question

MICROBIAL ECOLOGY:

The next set of methods refers to nucleic acid probes. What are these, and how do they work? Select all that are true

	The term refers to DNA or RNA oligonucleotide complementary to a sequence in a target gene.
	The probe can be used to identify organisms containing a nucleic acid sequence complementary to the probe.
	Oligonucleotide probes can be made fluorescent with certain dyes.
	The probes can be used in the method called FISH.

Answer 1

The next set of methods refers to nucleic acid probes. What are these, and how do they work

correct options regarding probes are as follows :

• The term refers to DNA or RNA oligonucleotide complementary to a sequence in a target gene.
• The probe can be used to identify organisms containing a nucleic acid sequence complementary to the probe.
• Oligonucleotide probes can be made fluorescent with certain dyes.
• The probes can be used in the method called FISH.

(probes are small sequence known, synthetic segments of nucleic acids. which are used for to detect specific sequence of DNA or RNA in genome of organism. probes are complementary to target sequence of DNA or RNA which bind/hybridies only matching sequence. to detect hybridization probes are labeled with radioactive isotopes or fluorescent dye. one of example of use of probe in hybridization is FISH (Fluorescent In- Situ Hybridization) technique. )