Hemocytometer Cell Count Calculation
1,2,3,4 = WBC Counting Squares. Each has a volume of 0.1ul.
A,B,C,D,E = RBC Counting Squares. Each has a volume of 0.004ul.
Diluting fluid used
(Isoton, spinal diluting fluid, methlene blue, etc) Amount of specimen) (Amount of diluent) Dilution Factor
Total # of Squares Counted _______ Size of squares counted (Circle one): Large (0.1) WBC or Small (0.004) RBC
Homework Answers
1. Diluting Fluid used
Example
Dilution Factor = Amount specimen * amount of diluent
Dilution Factor = Final Volume / Volume from stock
= 1 ml / 2 ml
Dilution factor = 2.000
2. Total number of Square counted = 16 ( Large )
WBC count = 16* 0.1
= 1.6 cells / cubic millimeter of blood cells
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