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L LLL Led Ex 5-12: Starch Hydrolysis extracellular enzymes • What is the product of starch hydrolysis? • What are the enzymes
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Ans) Whenever starch (polysaccharides) molecules undergo hydrolysis, it forms either monosaccharides, disaccharides or trisaccharides. The end products depends on the strength of enzymes used and the common enzymes are, α-Amylase, which produces the disaccharide maltose and the trisaccharide maltotriose.

- Enzymes involved in the processing of starch to sugars. The commercial processing of starch to mono- and oligosaccharides depends on the availability of three major enzymes — glucoamylase, alpha-amylase and glucose isomerase.

- Use of starch is accomplished by an enzyme called alpha-amylase. A medium containing starch is used. After inoculation and overnight incubation, iodine reagent is added to detect the presence of starch. Iodine reagent complexes with starch to form a blue-black color in the culture medium.

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