For an enzyme catalyzed reaction, the initial rate R. was determined at each initial concentration of...
Enzyme Kinetics Problem The initial rate for an enzyme-catalyzed reaction has been determined at a number of substrate concentrations. Data are given below: 5 27 23 65 1. Estimate V and K from a Michaelis-Menten graph of V versus [S] 2. Use a Lineweaver-Burk plot to analyze the same data. a. Determine V and Ka from the Lineweaver-Burk BONUS: If the total enzyme concentration was I nmol/L, what is K? Enzyme Kinetics Problem The initial rate for an enzyme-catalyzed reaction...
The initial rate, V, of an enzyme catalyzed reaction varies with substrate concentration as follows: 106 x Initial rate, Ms SJ, M 0.020 0.585 0.004 0.495 0.002 0.392 0.001 0.312 0.250 0.00066 Determine Vmax and Km for this reaction
Under what circumstances does an enzyme catalyzed reaction rate resemble a non-enzyme catalyzed reaction? At very low concentrations of substrate (Km is greater than S) the Michaelis-Menton equation can be simplified to? At very high concentrations of substrate, the Michaelis-Menton equation can be simplified to? How do you determine the initial rate of reaction
The following data has been obtained for an enzyme-catalyzed reaction with two different initial enzyme concentrations of 0.025g/L and 0.015g/L. V (g/L.min) [S] (g/L) Eo = 0.025g/L Ep = 0.015g/L 56.8 1.75 1.05 28.4 1.58 0.97 19.1 1.47 0.89 14.3 1.38 0.83 11.4 1.31 0.77 9.4 1.22 0.73 8.2 1.15 0.69 7.1 1.08 0.66 (a) Using the Lineweaver-Burke plot, determine Km and Vm for Eo = 0.025g/L (b) Using the Lineweaver-Burke plot, determine Km and Vm for Ep = 0.015g/L...
8. The initial rate for an enzyme-catalyzed reaction has been deter mined at a number of substrate concentrations. Data are as follows: (Sl (pmol/L) (pmol/L) min) 65 102 120 135 200 (a) Estimate Vmax and Ky from a direct graph of y versus (SI (these data are plotted in Figure 11.246). Do you find difficulties in get- ting clear answers? (b) Now use a Lineweaver-Burk plot to analyze the same data. Does this work better? 8. The initial rate for...
Initial rates of an enzyme-catalyzed reaction for various substrate concentrations are listed in the table below. From this data determine Km and Vm S(M)v (HM/min) 4.1x103 9.5x104 5.2x104 1.03x104 4.9x10-5 1.06x10 5.1x10-6 173 125 106 80 67 43