Homework Answers
The mechanism for triosephosphate isomerase:
In the first step, carboxylate anion abstract the acidic hydrogen near to aldehyde group which will give Enediol intermediate. Then keto-enol tautomerism takes place which will give the desired product.
In the reverse reaction also same mechanical steps repeats. First carboxylate anion abstract the acidic hydrogen near to the keto group which will give Enediol intermediate. Then keto-enol tautomerism takes place which will give the desired product.
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2. (12 points) Draw the mechanism for triose phosphate isomerase using arrow pushing. The enzyme contains...
2. Draw a detailed arrow pushing mechanism for the following transformation (3 points): i 1)^_MGBT OH...
2. Draw a detailed arrow pushing mechanism for the following transformation (3 points): i 1)^_MGBT OH H2) H20
12. In the following reaction: a) Write a detailed arrow pushing mechanism showing the stereochemistry (12...
12. In the following reaction: a) Write a detailed arrow pushing mechanism showing the stereochemistry (12 pts) HO: HO. ÖH Do not use these structures. Redraw them below and show OH every step. If an intermediate is common draw it twice!!! start your mechanism below the dotted line!!!
2. Using the arrow-pushing formalism, draw the mechanism for the transformation of your phosphine into the...
2. Using the arrow-pushing formalism, draw the mechanism for the transformation of your phosphine into the phosphonium salt you observed by HP NMR. 3. A brief written component answering the following questions. a. Based on your collected data, is 1-bromobutane or 2-bromobutane a more active electrophile? How do you explain this observation? b. Based on your collected data, is PPh or P(Bu) a more active nucleophile? How do you explain this observation?
41. points) a) Draw the catalytic triad as it appears in the active site of any...
41. points) a) Draw the catalytic triad as it appears in the active site of any serine protease: b) The following hexapeptide was cut with trypsin. thr-val-glu-lys-his-gin Using the single letter amino acid code, write the sequence of the ping pong reaction in the boxes provided below. Enzyme -Enzyme Double-displacement reaction c) You mutate the S1 pocket of trypsin from Glutamate to Isoleucine. What are the two new products of the digestion of the hexapeptide in part b (still use...
2. The table below contains data on the enzyme activity of mannose-6-phosphate isomerase (MPI) fo...
2. The table below contains data on the enzyme activity of mannose-6-phosphate isomerase (MPI) for three different genotypes (SS, FS, FF) in the amphipod crustacean Platorchestia platensis. Because the effects of sex are not known, specimens were classified by sex. Conduct a two-way ANOVA on this dataset. Test for effect of genotype, the effect of sex, and whether there is an interaction effect between two factors. Show all calculations and state your conclusions. FS Females 3.633.57 3.93 2.894.14.26 3.22 3.345.02...
12) Which of the following types of enzyme carries out phosphorylation of a protein? (a) isomerase...
12) Which of the following types of enzyme carries out phosphorylation of a protein? (a) isomerase (b) kinase (c) phosphodiesterase (d) lyase (e) hydrolase 13) Flavin adenine nucleotide is a coenzyme for which of the following? (a) monoamine oxidase (b) trypsin (c) chymotrypsin (d) Na+ K+ ATPase (e) all of the above 14) A noncompetitive inhibitor (a) binds only to [ES] (b) binds either [E] or [ES] (c) binds only to [E] (d) binds only to [S] (15) Which of...
2. (12 points, 6 points each) Give the arrow pushing mechanism for To of the following...
2. (12 points, 6 points each) Give the arrow pushing mechanism for To of the following reactions. Draw the resonance structures of the intermediate. You MUST include the mechanism for the formation of the electrophile for electrophilic substitution reactions. Do not combine steps and be sure to show arrows and charges for all steps. Gie Twoyo.coc.c.m.m.ci.e groded..ay are wotcleurly marked hwiti only gade wor A. HNO, H,So B. 1. AICI 2. H2o C. OzN NO2 NaOCH2CH 02N. heat N/A D....
2. (a) (i) Draw an arrow-pushing mechanism that illustrates the electron flow in the acid-base reaction...
2. (a) (i) Draw an arrow-pushing mechanism that illustrates the electron flow in the acid-base reaction below. Use the structures shown below in your arrow-pushing mechanism. (4pts for correct mechanism). (ii) Using pKa values indicated in Table 1.8 (round to nearest whole number) and estimate the equilibrium constant for this reaction (Keq). Show work which led to this value. (3pts for work, 1pt for correct Keq indicated). (iii) Draw a base (assuming the same acid provided below) that would reverse...
2. The reaction below is catalyzed by the CK enzyme: NH O | NHA | СК...
2. The reaction below is catalyzed by the CK enzyme: NH O | NHA | СК O= H₂N Ņ coo eo T N con + ATP + ADP Below is a picture of the active site for this enzyme. The red atoms are the amino acids in the CK protein. The blue structure is the substrate, the orange is the phosphate end of ATP. All dashed lines are showing interactions and the curved arrows are showing the mechanism. The wavy...
3. (2 points) Provide a mechanism, using curved arrow notation, for the formation of glycine and...
3. (2 points) Provide a mechanism, using curved arrow notation, for the formation of glycine and acetaldehyde from threonine. This process occurs by a PLP-dependent enzyme, you may begin your mechanism with the "PLP-primed" enzyme. Be sure to pay careful attention to stereochemistry throughout your mechanism. You may abbreviate any iminium formation, iminium hydrolysis, and transaldimination steps in your mechanism by the conventions shown in lecture. H OH H Me Oo Me H3N H Il acetaldehyde glycine threonine
2. Draw a detailed arrow pushing mechanism for the following transformation (3 points): i 1)^_MGBT OH H2) H20
12. In the following reaction: a) Write a detailed arrow pushing mechanism showing the stereochemistry (12 pts) HO: HO. ÖH Do not use these structures. Redraw them below and show OH every step. If an intermediate is common draw it twice!!! start your mechanism below the dotted line!!!
2. Using the arrow-pushing formalism, draw the mechanism for the transformation of your phosphine into the phosphonium salt you observed by HP NMR. 3. A brief written component answering the following questions. a. Based on your collected data, is 1-bromobutane or 2-bromobutane a more active electrophile? How do you explain this observation? b. Based on your collected data, is PPh or P(Bu) a more active nucleophile? How do you explain this observation?
41. points) a) Draw the catalytic triad as it appears in the active site of any serine protease: b) The following hexapeptide was cut with trypsin. thr-val-glu-lys-his-gin Using the single letter amino acid code, write the sequence of the ping pong reaction in the boxes provided below. Enzyme -Enzyme Double-displacement reaction c) You mutate the S1 pocket of trypsin from Glutamate to Isoleucine. What are the two new products of the digestion of the hexapeptide in part b (still use...
2. The table below contains data on the enzyme activity of mannose-6-phosphate isomerase (MPI) for three different genotypes (SS, FS, FF) in the amphipod crustacean Platorchestia platensis. Because the effects of sex are not known, specimens were classified by sex. Conduct a two-way ANOVA on this dataset. Test for effect of genotype, the effect of sex, and whether there is an interaction effect between two factors. Show all calculations and state your conclusions. FS Females 3.633.57 3.93 2.894.14.26 3.22 3.345.02...
2. (12 points, 6 points each) Give the arrow pushing mechanism for To of the following reactions. Draw the resonance structures of the intermediate. You MUST include the mechanism for the formation of the electrophile for electrophilic substitution reactions. Do not combine steps and be sure to show arrows and charges for all steps. Gie Twoyo.coc.c.m.m.ci.e groded..ay are wotcleurly marked hwiti only gade wor A. HNO, H,So B. 1. AICI 2. H2o C. OzN NO2 NaOCH2CH 02N. heat N/A D....
2. (a) (i) Draw an arrow-pushing mechanism that illustrates the electron flow in the acid-base reaction below. Use the structures shown below in your arrow-pushing mechanism. (4pts for correct mechanism). (ii) Using pKa values indicated in Table 1.8 (round to nearest whole number) and estimate the equilibrium constant for this reaction (Keq). Show work which led to this value. (3pts for work, 1pt for correct Keq indicated). (iii) Draw a base (assuming the same acid provided below) that would reverse...
2. The reaction below is catalyzed by the CK enzyme: NH O | NHA | СК O= H₂N Ņ coo eo T N con + ATP + ADP Below is a picture of the active site for this enzyme. The red atoms are the amino acids in the CK protein. The blue structure is the substrate, the orange is the phosphate end of ATP. All dashed lines are showing interactions and the curved arrows are showing the mechanism. The wavy...
3. (2 points) Provide a mechanism, using curved arrow notation, for the formation of glycine and acetaldehyde from threonine. This process occurs by a PLP-dependent enzyme, you may begin your mechanism with the "PLP-primed" enzyme. Be sure to pay careful attention to stereochemistry throughout your mechanism. You may abbreviate any iminium formation, iminium hydrolysis, and transaldimination steps in your mechanism by the conventions shown in lecture. H OH H Me Oo Me H3N H Il acetaldehyde glycine threonine
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