In the spinach extraction lab by chromatography, why is β-carotene the first pigment to be eluted...
In the spinach extraction lab by chromatography, why is β-carotene the first pigment to be eluted during the chromatography column? Explain your answer.
Homework Answers
Answer : Beta - carotene eluted out first during the chromatography column , this is because Beta - carotene is a non polar compound . And the solvent choose is hexane which is also non polar solvent . So, non polar compound dissolve in non polar solvent and eluted out first .
After that we increase the polarity of solvent by adding ethanol or ethyl acetate in hexane to elute other components also .
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In the spinach extraction lab by chromatography, why is
β-carotene the first pigment to be eluted...
LAB 6B PRELAB QUESTIONS To be turned in at the beginning of each lab period. You...
LAB 6B PRELAB QUESTIONS To be turned in at the beginning of each lab period. You will not be allowed to proceed without completing this section. Name: O-chem prela Day/Time: 1. Why is it important to have a uniform silica layer (even top and no bubbles) in column chromatography? 2. What is the purpose of using ethanol when mashing spinach leaves before extraction with dichloromethane (DCM)? 3. What percent of B-carotene can you expect spinach leaves to contain? Cite your...
Chromatographic features comparison: Predict the relative Rf values for the pigments, lycopene and B-carotene, on a...
Chromatographic features comparison: Predict the relative Rf values for the pigments, lycopene and B-carotene, on a TLC plate. Which pigment will elute firsr during column chromatography? explain in detail.
troduction: What is Carotene? Where is it found? How is it related to Vitamin A? ice...
troduction: What is Carotene? Where is it found? How is it related to Vitamin A? ice C6B5 him What happens if there is a deficiency in vitamin A? Methods: Don't copy out the entire procedure. Explain the procedure in the handout in your own words, and describe any changes you made to the procedure 1. 2, 3. 4. Include the table of the results for the absorbance values for all samples. show the calculations of the μg carotene. Graph absorbance...
Page 1 of 2 Exp. 3 Isolation of Caffeine from Instant Coffee: Extraction and Thin Layer Chromatography Please provi...
Page 1 of 2 Exp. 3 Isolation of Caffeine from Instant Coffee: Extraction and Thin Layer Chromatography Please provide each question you answer. Pre-Lab 1. Explain what a "partition or distribution" coefficient is. Use a dra coefficient of 3. 2. Explain how centrifugation works and critical safety consideration 3. Explain what anhydrous calcium chloride is and what it does in thi 4. Explain how thin layer chromatography works, what an Rt signifie 5. If the above squares are the display...
these questions are in reference to gel filtration chromatography Fost lab questions on lab 8: Analysis...
these questions are in reference to gel filtration
chromatography
Fost lab questions on lab 8: Analysis of Laboratory Results 1. If the following mix of molecules were parified using sine exclusion chromatography, what would be the 6 WI onder in which the molecules pass chrough the opening in the bottom of the column? Mixture containingR hemoglobin, 65,000 daltons; myoglobin, 17,000 daltons; myosin, 180,000 daltons. rhe tw First molecule to appear Second molecule to appear Third molecule to appear 2. Ifa...
Briefly explain how chromatography technique works. What type of chromatography is being used in this lab...
Briefly explain how chromatography technique works. What type of chromatography is being used in this lab activity? ___________________________________________________ __________________________________ What are the mobile phase and stationary phase substances/Compounds, which are used in this experiment? Briefly explain the purpose of each phase. ____________________________ ______________________________________ If the following mix of molecules were purified using size exclusion chromatography, what would be the order in which the molecules pass through the opening in the bottom of the column? Mixture containing: Hemoglobin, 65,000 Daltons; Ribonuclease...
Name:_ Post-Lab Questions You're the TA in an organic lab and the students are running a...
Name:_ Post-Lab Questions You're the TA in an organic lab and the students are running a column to separate the following compounds: methyl cinnamon trans-cinnamic acid HW 1. Student A calls you over and explains that after running his column and showing you the TLC of fractions 1-5 which only have one spot by UV. He combined these fractions, removed the solvent and took a melting point. He is worried something is wrong because he got 38°C and not 133°C....
Name:_ Post-Lab Questions You're the TA in an organic lab and the students are running a...
Name:_ Post-Lab Questions You're the TA in an organic lab and the students are running a column to separate the following compounds: methyl cinnamon trans-cinnamic acid HW 1. Student A calls you over and explains that after running his column and showing you the TLC of fractions 1-5 which only have one spot by UV. He combined these fractions, removed the solvent and took a melting point. He is worried something is wrong because he got 38°C and not 133°C....
photosynthesis lab yes we are using chromatography Photosynthesis lab sheet:2 (0) . Protected View Saved Search...
photosynthesis lab
yes
we are using chromatography
Photosynthesis lab sheet:2 (0) . Protected View Saved Search A Inni Massoud esign Layout References Mailings Review View Help the Internet can contain viruses. Unless you need to edit, it's safer to stay in Protected View Enable Editing ed credentials are out of date. Please sign in as I Massoud ebnet.org so we can verity your subscription Sign in Photosynthesis A. 1. Chromatography Lab Hypothesis Independent variable: Dependent variables Constant variables: Experimental group...
LAB 2A SEPARATION OF LIQUIDS BY SIMPLE DISTILLATION ANALYSIS BY GAS CHROMATOGRAPHY LAB 2A Pre-Lab Questions...
LAB 2A SEPARATION OF LIQUIDS BY SIMPLE DISTILLATION ANALYSIS BY GAS CHROMATOGRAPHY LAB 2A Pre-Lab Questions NAME DATE LAB SECTION Answer the following questions in the pre-lab section of your notebook. 1. Which compound, acetone or 1-propanol, has the higher boiling point? Why? 2. Do you expect acetone to be soluble in 1-propanol? Why or why not? Model: For this question, you will be graded on completion, rather than correctness of the model. In the post-lab, you will be asked...
LAB 6B PRELAB QUESTIONS To be turned in at the beginning of each lab period. You will not be allowed to proceed without completing this section. Name: O-chem prela Day/Time: 1. Why is it important to have a uniform silica layer (even top and no bubbles) in column chromatography? 2. What is the purpose of using ethanol when mashing spinach leaves before extraction with dichloromethane (DCM)? 3. What percent of B-carotene can you expect spinach leaves to contain? Cite your...
troduction: What is Carotene? Where is it found? How is it related to Vitamin A? ice C6B5 him What happens if there is a deficiency in vitamin A? Methods: Don't copy out the entire procedure. Explain the procedure in the handout in your own words, and describe any changes you made to the procedure 1. 2, 3. 4. Include the table of the results for the absorbance values for all samples. show the calculations of the μg carotene. Graph absorbance...
Page 1 of 2 Exp. 3 Isolation of Caffeine from Instant Coffee: Extraction and Thin Layer Chromatography Please provide each question you answer. Pre-Lab 1. Explain what a "partition or distribution" coefficient is. Use a dra coefficient of 3. 2. Explain how centrifugation works and critical safety consideration 3. Explain what anhydrous calcium chloride is and what it does in thi 4. Explain how thin layer chromatography works, what an Rt signifie 5. If the above squares are the display...
these questions are in reference to gel filtration
chromatography
Fost lab questions on lab 8: Analysis of Laboratory Results 1. If the following mix of molecules were parified using sine exclusion chromatography, what would be the 6 WI onder in which the molecules pass chrough the opening in the bottom of the column? Mixture containingR hemoglobin, 65,000 daltons; myoglobin, 17,000 daltons; myosin, 180,000 daltons. rhe tw First molecule to appear Second molecule to appear Third molecule to appear 2. Ifa...
Briefly explain how chromatography technique works. What type of chromatography is being used in this lab activity? ___________________________________________________ __________________________________ What are the mobile phase and stationary phase substances/Compounds, which are used in this experiment? Briefly explain the purpose of each phase. ____________________________ ______________________________________ If the following mix of molecules were purified using size exclusion chromatography, what would be the order in which the molecules pass through the opening in the bottom of the column? Mixture containing: Hemoglobin, 65,000 Daltons; Ribonuclease...
Name:_ Post-Lab Questions You're the TA in an organic lab and the students are running a column to separate the following compounds: methyl cinnamon trans-cinnamic acid HW 1. Student A calls you over and explains that after running his column and showing you the TLC of fractions 1-5 which only have one spot by UV. He combined these fractions, removed the solvent and took a melting point. He is worried something is wrong because he got 38°C and not 133°C....
Name:_ Post-Lab Questions You're the TA in an organic lab and the students are running a column to separate the following compounds: methyl cinnamon trans-cinnamic acid HW 1. Student A calls you over and explains that after running his column and showing you the TLC of fractions 1-5 which only have one spot by UV. He combined these fractions, removed the solvent and took a melting point. He is worried something is wrong because he got 38°C and not 133°C....
photosynthesis lab
yes
we are using chromatography
Photosynthesis lab sheet:2 (0) . Protected View Saved Search A Inni Massoud esign Layout References Mailings Review View Help the Internet can contain viruses. Unless you need to edit, it's safer to stay in Protected View Enable Editing ed credentials are out of date. Please sign in as I Massoud ebnet.org so we can verity your subscription Sign in Photosynthesis A. 1. Chromatography Lab Hypothesis Independent variable: Dependent variables Constant variables: Experimental group...
LAB 2A SEPARATION OF LIQUIDS BY SIMPLE DISTILLATION ANALYSIS BY GAS CHROMATOGRAPHY LAB 2A Pre-Lab Questions NAME DATE LAB SECTION Answer the following questions in the pre-lab section of your notebook. 1. Which compound, acetone or 1-propanol, has the higher boiling point? Why? 2. Do you expect acetone to be soluble in 1-propanol? Why or why not? Model: For this question, you will be graded on completion, rather than correctness of the model. In the post-lab, you will be asked...
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