Is it possible for a Staphylococcus spp. isolate to be slide coagulase negative and tube coagulase positive? Explain your answer
Yes, it is possible for Staphylococcus spp.isolate to be slide coagulase negative and tube coagulase positive.
Coagulase-negative staphylococci (CoNS) are a type of staph bacteria that commonly live on a person's skin. ... However, the bacteria can cause infections when present in large amounts, or when present in the bloodstream. Doctors often divide staph bacteria into coagulase-positive and coagulase-negative types.
Coagulase is a protein enzyme produced by several microorganisms that enables the conversion of fibrinogen to fibrin. In the laboratory, it is used to distinguish between different types of Staphylococcus isolates. ... However it is now known that not all S. aureus are coagulase-positive.
However, the tube coagulase gene can be present in other staphylococci, such as S intermedius and S hyicus. Therefore, it is not absolutely specific for S aureus. ...Tube coagulase negative but slide coagulase positive staphylococci include S lugdunensis and S schleiferi.
for example, To ascertain the clinical importance of a strain of slide coagulase positive but tube coagulase negative Staphylococcus species isolated from the blood culture of a 43 year old patient with refractory anaemia with excessive blasts in transformation who had neutropenic fever. The isolate was investigated phenotypically by standard biochemical methods using conventional biochemical tests and two commercially available systems, the Vitek (GPI) and API (Staph) systems. Genotypically, the 16S ribosomal RNA (rRNA) gene of the bacteria was amplified by the polymerase chain reaction (PCR) and sequenced. The sequence of the PCR product was compared with known 16S rRNA gene sequences in the GenBank by multiple sequence alignment. Conventional biochemical tests did not reveal a pattern resembling a known Staphylococcus species. The Vitek system (GPI) showed that it was 94% S. simulans and 3% S. haemolyticus, whereas the API system (Staph) showed that it was 86.8% S. aureus and 5.1% S. warneri. 16S rRNA gene sequencing showed that there was a 0 base difference between the isolate and S. aureus, 28 base difference between the isolate and S. lugdunensis, 39 base difference between the isolate and S. schleiferi, 21 base difference between the isolate and S. haemolyticus, 41 base difference between the isolate and S. simulans, and 23 base difference between the isolate and S. warneri, indicating that the isolate was a strain of S. aureus. Vancomycin was subsequently prescribed and blood cultures taken four days after the start of treatment were negative. 16S rRNA gene sequencing was useful in ascertaining the clinical importance of the strain of slide coagulase positive but tube coagulase negative Staphylococcus species isolated from blood culture and allowing appropriate management.
Is it possible for a Staphylococcus spp. isolate to be slide coagulase negative and tube coagulase...
STUDY QUESTIONS: STAPHYLOcocc 1. Why would you perform a coagulase test on a bacterial isolate that tested positive for mannitol salt agar fermentation? 2. Would a catalase positive test always signify the presence of Staphylococci spp.? 3. What is the medical significance of a coagulase positive test result?
RESULTS Pathogenic Potential Coagulase Production Staphylococcus aureus Wall Staphylococcus epidermidis Enterococcus faecalis 169 PARTY: Identification of Gram Positive and Gram Negative Bacteria tab Activity 82 Coagulase Test: Post-Lab
Culture site: stitch abscess white, medium sized colony beta hemolysis catalase – positive slide coagulase – positive tube coagulase- positive A disk – negative CAMP test – negative Bile esculin – positive NaCl – positive Optochin disk – negative Bile solubility – negative PYR – negative id the organism
microbiology QUESTIONS Consider the controls. a. Are the uninoculated tube and the saline on the slide positive or negative controls? egatwe b. What information is provided by the uninoculated tube in the tube test? What information is provided by the saline solution in the slide test? d. Why is it a good idea to runa positive control in both the slide and tube tests? it more imfortant to uca froch culturas in the coagulase tect than in a test me
May you please put this in layman's terms? ABSTRACT Coagulase-negative staphylococci (CoNS) and Staphylococcus aureus are part of the natural flora of humans and other mammals. We found that spent media from the CoNS species Staphylococcus caprae can inhibit agr-mediated quorum sensing by all classes of S. aureus. A biochemical assessment of the inhibitory activity suggested that the S. caprae autoinducing peptide (AIP) was responsible, and mass spectrometric analysis identified the S. caprae AIP as an eight-residue peptide (YSTCSYYF). Using...
In a slide trombone, the tube increases in length as the musician extends the slide. How does extending the slide affect the fundamental frequency of the trombone? Explain. A person sitting in a parked car hears an approaching ambulance siren at a frequency f, and as it passes him and moves away he hears a frequency f'. How does the actual frequency of the source compare to f and f'? Explain your reasoning. Your options are: Greater than f' but...
Microbiology - Gram Positive (ONLY answer) [Q1] Enterococci and Streptococci are Gram-negative organisms. - True - False [Q2] Which of the following is an important test that should be done first before identifying an unknown to be Staphylococcus or Streptococcus? - Catalase - Coagulase - Blood Agar - A Disc Test [Q3] Which of the following media is used for antibiotic sensitivity testing? - Mannitol - Mueller Hinton - P Disc Test - A Disc Test
Problem 10.65 In an amusement park water slide, people slide down an essentially frictionless tube. The top of the slide is 3.2m above the bottom where they exit the slide, moving horizontally, 1.2m above a swimming pool. Part A What horizontal distance do they travel from the exit point before hitting the water? Express your answer to two significant figures and include the appropriate units. d =
please help me out! Negative and positive controls Tube 1: 1 ml Catalase + 3 ml distilled water Tube 2: 1 ml Catalase + 3 ml sucrose Tube 3: 1 ml Catalase + 3 ml H,O, Positive test produces bubbles; negative reaction does not bubble Question #1: State the NUMBER of the ONE tube that is the negative control: Question #2: Explain why no bubbles will appear in Tube 2: Effect of concentration - Tube 4: 1 ml catalase +...
I need a help to solve the questions 2, 5,6, 8 and 10. BACTERIOLOGY LAB PRACTICAL You are welcome to use your lab exercises, class notes or textbook to answer the following questions. Please remember that your isolate is something that we worked with at some point in the semester, so it should look familiar. You are welcome to take this part of the lab practical home to work on, but please have it ready to turn in at the...