how would you prepare a 1:1000 dilution of a standard solution
Ans) To make a 1:100 dilution,
one part of the solution is mixed with 99 parts of additional solvent. Mixing 100 µL of a stock solution with 900 µL of water makes a 1:10 dilution. The final volume of the diluted sample is 1000 µL (1 mL) and the concentration is 1/10 that of the original solution.
How would you prepare 1.5 ml of a 1:1000 dilution of the CB2 primary antibody (1° Ab CB2) in 1% milk solution if 1.5 μl is provided in a tube. Specifically, how much 1% Milk in DPBS will you add to the tube?
How would you prepare a 1/100 dilution (of a concentrated stock solution) in a final vol of 100 ml? 2.5 L? 87 ml? (ie how much diluent and how much of your concentrated stock)?
How would you make a 1:1000 dilution of E. coli?
Explain how you would make a 1/1000 dilution of a bacterial suspension if you only had 3 ml aliquots of BHI broth (diluent) available. What size pipetman will you need to use to make this dilution?
How would you prepare a 1:500 dilution? You cannot use more than 10mL per tube.
describe quantitatively and qualitatively how you would prepare 1000. mL of a 2.00 M solution of nitric acid from a 18.0 M H2SO4 stock solution
1. How would you prepare 125 ml of a 10% (v/v) solution of acetic acid in H2O? 2. How would you prepare 60 ml of a 5% (W/V) NaCl solution in H20? 3. How would you prepare 3 ml of 82% solution A and 18% solution B? 4. 5 ml of a 2.0 mg/ml protein solution contains mg protein. a. If 1.0 ug/ml of pigment X shows an absorbance of 0.059 at 370nm, then what is the concentration of a...
You are given a 1 M solution of sodium chloride. How will you prepare 1000 μL of 0.2 M solution of sodium chloride?
You are given a 1 M solution of sodium chloride. How will you prepare 1000 μL of 0.2 M solution of sodium chloride?
1) How would you prepare 500mL of working TBE buffer if the stock solution was purchased as 25X TBE? 2) 2mL of a serum sample was added to 18mL of phosphate buffered saline (PBS) in Tube 1. 10mL of Tube 1 was added to 40mL of PBS in Tube What is the dilution of serum in Tube 2? 3) A student needed to prepare 500mL of 1X TAE buffer to run a QC gel. The stock solution in the lab...