How would you prepare a 1:500 dilution? You cannot use more than 10mL per tube.
How would you prepare a 1:500 dilution? You cannot use more than 10mL per tube.
How would you prepare 1.5 ml of a 1:1000 dilution of the CB2 primary antibody (1° Ab CB2) in 1% milk solution if 1.5 μl is provided in a tube. Specifically, how much 1% Milk in DPBS will you add to the tube?
You have 5ml of an overnight culture (2x108 cells per ml). How would you prepare a series of 1 or 10ml dilutions (maximum dilution 1:100) to obtain a final 200 ml culture at 1x103 cell/ml? Provide work for each step.
1) How would you prepare 500mL of working TBE buffer if the stock solution was purchased as 25X TBE? 2) 2mL of a serum sample was added to 18mL of phosphate buffered saline (PBS) in Tube 1. 10mL of Tube 1 was added to 40mL of PBS in Tube What is the dilution of serum in Tube 2? 3) A student needed to prepare 500mL of 1X TAE buffer to run a QC gel. The stock solution in the lab...
how would you prepare a 1:1000 dilution of a standard solution
How would you prepare a 2.5m solution of HNO3 from 10mL of 14m HNO3 ?
How would you prepare a 1/100 dilution (of a concentrated stock solution) in a final vol of 100 ml? 2.5 L? 87 ml? (ie how much diluent and how much of your concentrated stock)?
Dilution, help?? 5. You add 25uls of antibody to 475 uls of water - what dilution did you just make? 6. (3 pts) To obtain a countable plate to quantify your bacterial sample you first dilute the sample 1/500 in Tube 1 and then make a 1/25 dilution of this sample in Tube 2. If the total volume for your dilutions is 1 ml: a. How much water should be in Tube 1 and how much of your initial bacterial...
Your cell suspension is 6.3 x 10^8 cells /mL. Explain how to prepare a tube of 1 mL with cells at 2 x 10^5 cells/mL. Pipetting less than 1 μL is hard to do accurately. Do a dilution if less than 1μL After dilution,Now that you have your tube of 1 mL with cells at 2 x 10^5/mL, explain how to add 10^4 cells to each of 10 wells. Do you have enough cells?
Serial dilution problem You have a stock of 5% SDS. You take some of that stock and dilute it 4-fold in tube #1. the tubes hold 1.5ml. give an example of how much 5% stock and how much water you would use to make 4 fold dilution - volume of 5% stock ? - volume of water? - How much volume transferred? - if you took a sample out of tube 2 and did another 4 fold dilution, what concentration...
If you have 1.0 x 10 molecules in a tube and perform a 10-4 dilution, how many molecules will be in your new tube? 1000 molecules 10,000 molecules • 10 molecules 100 molecules