How would you prepare 1.5 ml of a 1:1000 dilution of the CB2 primary antibody (1°...
How would you prepare 1.5 ml of a 1:1000 dilution of the CB2 primary antibody (1° Ab CB2) in 1% milk solution if 1.5 μl is provided in a tube. Specifically, how much 1% Milk in DPBS will you add to the tube?
Homework Answers
Answer:
Here milk is the diluent,
Dilution factor= (aliquot vol + diluent vol)/aliquot vol
1000= (1.5uL+ diluent vol)/1.5uL
(1000 x 1.5)- 1.5= diluent volume
diluent volume= 1498.5uL
Add 1498.5uL of 1% milk to the 1.5uL antibody provided to you.
Add Answer to:
How would you prepare 1.5 ml of a 1:1000 dilution of the CB2
primary antibody (1°...
Dilution, help?? 5. You add 25uls of antibody to 475 uls of water - what dilution...
Dilution, help??
5. You add 25uls of antibody to 475 uls of water - what dilution did you just make? 6. (3 pts) To obtain a countable plate to quantify your bacterial sample you first dilute the sample 1/500 in Tube 1 and then make a 1/25 dilution of this sample in Tube 2. If the total volume for your dilutions is 1 ml: a. How much water should be in Tube 1 and how much of your initial bacterial...
D O BLO 1151CI UURI. 5) How much primary antibody do you need to add to...
D O BLO 1151CI UURI. 5) How much primary antibody do you need to add to 10mL of milk (Blocking) solution to get a 1:1000 concentration? There needs to be 10ul of the primary antibodies added to 10ml af mill to the
how would you prepare a 1:1000 dilution of a standard solution
how would you prepare a 1:1000 dilution of a standard solution
How would you prepare a 1/100 dilution (of a concentrated stock solution) in a final vol...
How would you prepare a 1/100 dilution (of a concentrated stock solution) in a final vol of 100 ml? 2.5 L? 87 ml? (ie how much diluent and how much of your concentrated stock)?
Your cell suspension is 6.3 x 10^8 cells /mL. Explain how to prepare a tube of...
Your cell suspension is 6.3 x 10^8 cells /mL. Explain how to prepare a tube of 1 mL with cells at 2 x 10^5 cells/mL. Pipetting less than 1 μL is hard to do accurately. Do a dilution if less than 1μL After dilution,Now that you have your tube of 1 mL with cells at 2 x 10^5/mL, explain how to add 10^4 cells to each of 10 wells. Do you have enough cells?
Dilution Practice Problem #5 You are given the following bacterial dilution scheme: ml 1 ml 1...
Dilution Practice Problem #5 You are given the following bacterial dilution scheme: ml 1 ml 1 ml 1 ml 1 ml CODILE 9ml 9ml 9ml 0.1 ml 0.1 ml colonies) (colonies colonies) Individual tube dilutions: BCD Dilution Factor: Total tube dilutions) IDE AB Plated dilution factor 1. Which plate is the countable plate? (30-300 countable range) 2. Calculate the titer of the original culture in cfu/ml.
Explain how you would make a 1/1000 dilution of a bacterial suspension if you only had...
Explain how you would make a 1/1000 dilution of a bacterial suspension if you only had 3 ml aliquots of BHI broth (diluent) available. What size pipetman will you need to use to make this dilution?
please show work so: 1 ml stock 10 ml final -0.5 mL of stock -5 ml...
please show work
so: 1 ml stock 10 ml final -0.5 mL of stock -5 ml final volume While I have used 1:10 as a standard the same rules apply for all dilution factors. Try these problems: x 0.5 X0.5 1. You need to make a 1:25 dilution factor. If you final volume is 25 mL this means that you need_ _mL of stock and mL of solvent to make your final volume of 25 mL. 2. If you needed...
b. 1 ml 1ml 「-I Final dilution 4(1 point) This represents a serial dilution to determine...
b. 1 ml 1ml 「-I Final dilution 4(1 point) This represents a serial dilution to determine viable bacterial counts. Indicate the final dilutions in each of the two tubes. Note that two different volumes of the cells intube #2 are plated onto agar plates A and B. Indicate the dilution factor that will be used to report the original cell concentration on a per mL basis. 1 ml 1 ml Final dilution in Tube #1 = Final dilution in Tube...
Part A You must prepare 600 mL of a disinfectant that requires a 1:8 dilution of...
Part A You must prepare 600 mL of a disinfectant that requires a 1:8 dilution of a stock solution. How much of the stock solution will be required? Express your answer to two significant figures and include the appropriate units. Value Units Submit Request Answer
Dilution, help??
5. You add 25uls of antibody to 475 uls of water - what dilution did you just make? 6. (3 pts) To obtain a countable plate to quantify your bacterial sample you first dilute the sample 1/500 in Tube 1 and then make a 1/25 dilution of this sample in Tube 2. If the total volume for your dilutions is 1 ml: a. How much water should be in Tube 1 and how much of your initial bacterial...
D O BLO 1151CI UURI. 5) How much primary antibody do you need to add to 10mL of milk (Blocking) solution to get a 1:1000 concentration? There needs to be 10ul of the primary antibodies added to 10ml af mill to the
Dilution Practice Problem #5 You are given the following bacterial dilution scheme: ml 1 ml 1 ml 1 ml 1 ml CODILE 9ml 9ml 9ml 0.1 ml 0.1 ml colonies) (colonies colonies) Individual tube dilutions: BCD Dilution Factor: Total tube dilutions) IDE AB Plated dilution factor 1. Which plate is the countable plate? (30-300 countable range) 2. Calculate the titer of the original culture in cfu/ml.
please show work
so: 1 ml stock 10 ml final -0.5 mL of stock -5 ml final volume While I have used 1:10 as a standard the same rules apply for all dilution factors. Try these problems: x 0.5 X0.5 1. You need to make a 1:25 dilution factor. If you final volume is 25 mL this means that you need_ _mL of stock and mL of solvent to make your final volume of 25 mL. 2. If you needed...
b. 1 ml 1ml 「-I Final dilution 4(1 point) This represents a serial dilution to determine viable bacterial counts. Indicate the final dilutions in each of the two tubes. Note that two different volumes of the cells intube #2 are plated onto agar plates A and B. Indicate the dilution factor that will be used to report the original cell concentration on a per mL basis. 1 ml 1 ml Final dilution in Tube #1 = Final dilution in Tube...
Part A You must prepare 600 mL of a disinfectant that requires a 1:8 dilution of a stock solution. How much of the stock solution will be required? Express your answer to two significant figures and include the appropriate units. Value Units Submit Request Answer
Most questions answered within 3 hours.
-
Computer Programming II CS141(Java)
Mention the appropriate relationship between following
classes:
HOD–StaffMember
Car–Ferrari
Student-Address
BankAccount–FixedAccount
House-Building...
asked 1 hour ago -
Assume one of your finals has 50 questions on it, and
lucky for you, it's all...
asked 3 hours ago -
Rice Products in Bangladesh
Business behavior is derived in large part from the basic cultural
environment...
asked 4 hours ago -
The following base sequence is found for a mRNA fragment from
wild-type E. coli: 5'- UAUCAGUAGAUAAUGUAACC-3'...
asked 5 hours ago -
For this exercise, round all regression parameters to three
decimal places.
One of the two tables...
asked 5 hours ago -
What is the 5% level of significance for mean = 3.60, standard
deviation = 0.94, and...
asked 5 hours ago -
Prior to beginning work on this discussion, please read the
article by Hayley Peterson, 15 Companies...
asked 5 hours ago -
Which pair of aqueous solutions, when mixed, will form a
precipitate?
A) NaNO3 and AgC2H3O2
B)...
asked 6 hours ago -
1-Write an algorithm to get two numbers from the user (as
inputs) and calculate the sum...
asked 9 hours ago -
Define white-collar crime. What is the difference between
offender and offense-based definitions of white-collar crime? What...
asked 10 hours ago -
Consider a reaction which is 1st order with respect to A and 1st
order with respect...
asked 10 hours ago -
c++
The length of the hypotenuse of a right-angled triangle is the
square root of the...
asked 10 hours ago