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4. In the transfer technique procedure why is it important to cool the loop before obtaining an inocul btaining an inoculum o
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Answer: In the transfer technique procedure it is important to cool the loop before obtaining an inoculum of bacteria because as when the loop is hot and touces the media, it may create aerosol as well as due to hot loop, some of the bacteria starts to boil which have higher risk that can contaminates the air and enter to the respiratory tract.

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