please show work for both with labels and explainations this is an example of what is...
1.) Describe the goals of a Gel Filtration Chromotography Experiment??? 2.) Explain each key theoretical principle of a Gel Filtration Chromotography, and how they help acheive the goal???. 4.) Explain the key equations used in the Gel Filtration Chromotography experiment and the terms involved in the equation???? HI im trying to prepare for a lab/report and i have some questions i could use help with please :) over all having trouble seeing how everything ties together etc :) thank you...
You are dissatisfied with the purity of the final preparation of your HRP and decided to include ion-exchange (IEC) and gel-exclusion chromatography (GEC) steps in your purification protocol. From the literature, you found out the following info about HRP enzyme: Molecular weight: 40,000 Isoelectric point: 7.2 HRP is a very stable protein. 2: Design GEC purification experiment using the information in the appendix. Address and justify the following I. choice of GEC column II. choice of the buffer (if you...
Biochem help 2 14. What would be the net charge on the dipeptide Ser-His at pH- 6.04? (Choose the one best answer.) a) 1.5 b) +1; c) +0.5 d) 0 e) -0.5; 15. The pKa of a lysine side chain in a protein ending up on the outside of a globular protein has a different pKa than if the lysine is buried within the interior of a protein. What would be the expected pKa of a side chain of lysine...