Question

Compare and contrast using RNAi technology to trigger gene knockdown in Mice, C. elegans and Drosophila. Emphasize how the biology of each species guides different design principles.

Answer 1

DOMS" Page No. Date : Compare & contrast using RNA i technology to trigger Igene knockdown in mice, c-eleganse & Drospophila Emphasize how biology, g each speves guides diggecent design principles RNA i= Individual genes can be silenced by a mechanism called RNA interference CRNA i) - It is a mechanism by which small fragment of double stranded (ds) RNA whose sequence matches pact a genes sequence interfers with Cire silences) the expression & that gene. @ RNA i technology to tregger gene in mice > 1 RNA; - expressing construcs Toe protein Knockdown in mice , CIKKI gene) as target gene. At - And results shows that construct containing the I H I promoter are sensitive to the presence of prokaryotic Sequences & to transgene position effect, similar to IRNA pol I promoted driven constructs. I This is obsecved that variable expression level a transgenic siRNA among different tissues & D reduction of upto 80% in Ikk1 gene. © RNA; technology to trigger knock doron gene in oC-elegans → In nematode C. elegans, antisense RNA made in I vitro & injected into the worm could interfece with genes activity. And surprisingly control-sense RNA hed similar effect RNA; has natural functions including protection agains visas - RNAi is highly specific

In C. elegans to knockdown the expression 9 PAR 1 gene by antisense RNA, I obsecved Hlat similac loss egene expression- with sense RNA controls as well. double-stranded RNA in nematode is specific interference. Genetic interference is genetically heritable 0 RNAi technology to trigger gene in Drosophila je Specific gene silencing has been achieved in the embryo extracts of culture cells of Drosophilla > Lygates the embryo to demonstrate the cleavage g long as RNA strands into short interfering do RNA teagments (siRNA) a ~22 nucleotides Each species guids different design principles * O RNAi has been used for the functional knockdown g specific proteins in several experiments from cubrired cells. including mammals. Knockdown transgenic animals are produced by the introduction a RNAi construct in the cells. by lentiviral Vectors or pronuclear micro injection ② It is potentially applicable for decreasing the expression of the protein of interest. 4 RNAi usually results in partial inhibition q expression in Range 60 to goy.)