Question

No 1/ks] From this graph, explain Ez on Es which has more catalytic efficiency? And why? And if you increase the substrate concentration which one. E, OR E line go up or down a

Answer 1

Non competative inhibition - This type of inhibition is recognised by its characteristics effects on vmax noncompetective inhibition occurs when the inhibitors and substrute bind at difterne site of enzyme. The non-compitative inbibitwo can bind either free enzyme or the Es complex thereby preventing the reaction from occuring. D Effects on vmax - Nen competative inhibition cannot be overcome by the increasing the concentration of substrute. Theis non competative inhibitors decrease the vmes of the reaction 1) The Effeers on km - Noncomperative inhibitors do not interfere with the binding of substure to enzyme. This the enzyme show the same km in the presence or absence of the noncompetative inbibitors. 111) Effects on lineweare - Burke plot - Noncompetective inhibition is readily differntiated from comperative inhibitons by plotting No versus 1/Cs) and noting that max decrecue in presence of a non competitive inhibitors where as km is unchanged. - 1 Example of non competative inhibitors > End products of most of the naturally occuring metabolites pathway can inhibitod non compitative and reversibly by binding to a site on the enzyme molecules other than active site eg- L- Threonine dehydrature is inhibited ire. - isolecieine. Cyanide inhibits cytochrom oxidare where cyanide bind to the prosthetic group at the enzyme without affecting the main active site some time an inhibitors can acts both lik a competentive and non competative inhibitors for a perticalar substrute depend on neature of reaction eg malate dehydrogenare a mitochondrial enzyme Catalys is the reversible oxidation of malate to oriloacerat the presence of NADT.