Question

need help 7f and 7h

Figure 3: Reaction rate produced by Enzyme X with various inhibitor treatments Reaction rate 30 mM 30 mM 60 mM 60 mM substrate +100 60 mM 30 mM substrate +100 l Inhibitor substrate+ substrate+ Substrate substrate +100 ul Inhibitor +100 l Inhibitor no inhibitor no inhibitor pet inhibe hhthitohior

Answer 1

In general inhibitors are the substance which binds to enzyme and decreases its activity and hence decreases the rate of reaction.

7e.inhibitor B is a poor inhibitor of enzyme X

7f. B is a poor inhibitor as addition of this do not decreased the rate of forward reaction. Rate of reaction is same in the absence of inhibitor and in presence of inhibitor B.

7g. B is a competitive inhibitor.

7h. In case of a competitive inhibitor, the inhibitor remains in competition with the substrate for the active site of the enzyme, at low substrate concentration it reduces the rate of reaction. But in case of high concentration of substrate the inhibitor can be neglected, so the same Vmax and hence same reaction rate as the reaction without inhibitor can be obtained. This is not the case with Noncompetitive inhibitors as they do not bind the enzymes active site,