Question

QUESTION 1 OF 10 Please read Chapter 1 of the Lab Manual and the relevant Power Point lecture in detail, and answer the following question. Deadline for submission: April 2, 2020. Cell Division (Mitosis) Suppose that in an experiment you observed 113 cells in interphase, 24 cells in prophase, 9 cells in metaphase, 12 cells in anaphase, and 3 cells in telophase stages. If the complete cell cycle (i.e. Gi+S+G2+mitosis; see Figure 1-2 of the Lab Manual) requires 24 hours, calculate the average duration of each stage in the cycle. Show all calculations.

Suppose that in an experiment you observed 113 cells in interphase, 24 cells in prophase, 9 cells in metaphase, 12 cells in anaphase, and 3 cells in telophase stages. If the complete cell cycle (i.e. G1+S+G2+mitosis; see Figure 1-2 of the Lab Manual) requires 24 hours, calculate the average duration of each stage in the cycle. Show all calculations.

Answer 1

Prophase index= (P/(Total number of cells))*100

where, P is the number of cells in prophase

Prophase index= (24/(113+24+9+12+3)) x 100

= 14.9%

So the 14.9 % of the total cell cycle belongs to prophase

Duration of prophase= (14.9/100) x 24 hours

= 3.58 hours

Metaphase index= (M/(Total number of cells))*100

where, M is the number of cells in metaphase

Metaphase index= (9/(113+24+9+12+3)) x 100

= 5.59%

So the 5.59 % of the total cell cycle belongs to Metaphase

Duration of metaphase= (5.59/100) x 24 hours

= 1.34 hours

Anaphase index= (A/(Total number of cells))*100

where, A is the number of cells in Anaphase

Anaphase index= (12/(113+24+9+12+3)) x 100

= 7.45%

So the 7.45 % of the total cell cycle belongs to Anaphase

Duration of anaphase= (7.45/100) x 24 hours

= 1.79 hours

Telophase index= (T/(Total number of cells))*100

where, T is the number of cells in telophase

Telophase index= (3/(113+24+9+12+3)) x 100

= 1.86%

So the 1.86% % of the total cell cycle belongs to telophase

Duration of telophase= (1.86/100) x 24 hours

= 0.45 hours

So the total duration of mitosis is = (3.58+1.34+1.79+0.45)

= 7.16 hours

Therefore the time the cell spends in Interphase= (Total duration of cell cycle - Time spent in mitosis)

= (24 - 7.16)

= 16.84 hours