Calculate the number of grams of solid Tris-base (121.1 g/mol) required to prepare 250.0 mL of...
If you needed to make 500 mL of a TBE buffer (containing 0.1M Tris base, 0.1M Boric Acid, and 0.02M EDTA) how much of each of the following would you use: solid Tris base (121.1 g/mol); solid boric acid (61.8 g/mol); liquid stock solution of 0.50M EDTA?
Indicate the appropriate protocol below to prepare the required solution A) Solution 1 Preparation: Prepare: 1 liter of 2 Molar Tris From: solid Tris, MW = 121.1 g/mol Procedure: B) Solution 2 Preparation: Prepare: 1 liter of 0.01 mg/mL ascorbic acid From: solid ascorbic acid , MW = 176.1 g/mol Procedure: C) Solution 3 Preparation: Prepare: 1 liter of 100 mM Tris From: 1 Molar Tris stock solution Procedure: D) Solution 4 Preparation: Prepare: 1 liter of 50 mM CaCl2...
How much solute is required to make 600 ml of a 0.4M solution of Tris buffer. FW of Tris base = 121.1 grams.
How do you prepare 500 mL of 250 mM Tris base? (The molecular weight of Tris base is 121.14 g/mol)
How many grams of solid KCl are needed to prepare 250.0 mL of 0.235 M solution?
1) Solution Components A 1 mL 100 mM NaH2PO4 + 9 mL 100 mM Na2HPO4 B 5 mL 100 mM NaH2PO4 + 5 mL 100 mM Na2HPO4 C 9 mL 100 mM NaH2PO4 + 1 mL 100 mM Na2HPO4 D 10 mL 100 mM NaH2PO4 stock solution E 10 mL 100 mM Na2HPO4 stock solution F 10 mL distilled water pKa of phosphate: 6.8 How would you calculate the pH of each equation using the Henderson Hasselbalch equations? 2) Tris...
11. How many grams of solid KOH are needed to prepare 250.0 mL of 0.205 M KOH? O 0.820 g O 11.480g O 0.205 g O 0.0515 O 2.875 g
Grams solute 1.50 g NaCl Molarity/Titrations/stoichiometry Volume solution Molarity solution 100.0 mL 0.2104 M 250.0 mL 0.4345 M 25.0 mL 0.500 M CuCl2 20.0g MgBr2 50.0 mL 0.295 M Ni(NO3)2 2.5 g KNO3 0.325 M KNO3 10.0 g Fe(ClO3)3 0.408 M Fe(ClO3)3
Solutions practice problems. calculate how much of each ingredient you would need to make the solution CIRCLE YOUR FINAL ANSWERS. Be sure to include the correct units with your answer. Molecular weights: SDS-288.38 g/mol Tris (base) = 121.1 g/mol EDTA = 372.2 g/mol Sodium acetate 82.03 g/mol Tris maleate = 237.2 g/mol Magnesium sulfate heptahyrdate=246.5 g/mol Sodium hydroxide - 40.00 g/mol (can calculate the MW for simple compounds) 1) 200 mL of 25% SOS 2) 60 ml alkaline lysis buffer...
Calculate the grams of sodium acetate required to make 250.0 mL of a pH 5.50 buffer solution using 0.100 M acetic acid (Ka of 1.8x10-5).