How much solute is required to make 600 ml of a 0.4M solution of Tris buffer. FW of Tris base = 121.1 grams.
Volume = 600 mL = 600 / 1000 = 0.600 L
Moles = Molarity x volume = 0.4 M x 0.600 L
= 0.24 moles.
Mass of solute = Moles x Molar mass
= 0.24 moles x 121.1 g/mol
= 29.06 g.
Therefore, mass of solute = 29.06 g.
How much solute is required to make 600 ml of a 0.4M solution of Tris buffer....
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