What stain is the chromophore (the colored ion) in the following capsule stain procedure? Would it...
What stain is the chromophore (the colored ion) in the following capsule stain procedure? Would it be Congo Red or Acid fuchsin
Add 1 or 2 loops of bacteria to drop of Congo Red
Allow time air dry
Cover smear with acid-alcohol
rinse with water
Cover smear with acid fuchsin
rinse with water
blot dry
Homework Answers
It is a method of negative staining. In this Background is stained and capsule is not stained.
Congo red stains the background red. Now acid fuchsin stains the bacterial cells leaving capsule unstained.
So, capsule appears colourless.
Acid fuchsin stains the cells.
So, chromophore will be acid fuchsin.
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