Question

A U S D Foundations of Biology: Cell and Molecular Biology Laboratory Match the reagent in column A with its function in column B: A 1. Glysine solution a. Binds to amylose and give it blue color 2. Taq Polymerase b. Separates relatively larger proteins C. Acts as a reference that provides the information of the putative length of nucleotides d. Binds to hydrophobic parts of the proteins and stain 3. Primer 4. IKI (potassium iodide solution) them e. A natural constraint that keeps developing embryos in their embryogenic state f. a small piece of oligonucleotides that binds on DNA template 5. Comassie Blue reagent 6. Agarose Gel 7. Abscisic acid (ABA) g. Biological buffer 8. DNA ladder h. An enzyme that replicates the template DNA A (1-8) 2 1 3 4 5 6 7 8 ARO Match B (a-i) Laboratory N° 10 Signal Transduction: Antagonistic effects of Gibberellin (GA) and Abscisic Acid (ABA) on the Production of a-Amylase in Barley Seeds 1. (3pts) In an intact seed where embryc is not removed, where is the origin of Gibberellin (GA)? a. Aleurone layer c. Starchy endosperm/ Starch stores b. Embryo d Scutellum 2. (3pts) What's the effect abscisic acid(ABA) in lab 10? (aIt inhibits the activity of amylase C. It inhibits the production of GA b. It inhibits the decomposition of starch d. It inhibits the production of amylase 3. (9pts) To test the activity of amylase, you used reagent 4. (3pts) To quantify the result in determining amylase activity, which wavelength did you use in spectrophotometer? as substrate, and used for color development 1

Answer 1

1. Glysine solutions - g. Biological buffer

2. Taq polymerase - h. A enzyme that replicates tha template DNA

3. Primer - f. A small piece of oligonucleotides that binds on DNA.

4. IKI (potassium iodine solution) - a. Binds to amylose and give it blue colour

5. Commasie blue reagents - d. Binds to the hydrophobic parts of the proteins and stain them

6. Agarose gel - b. Separates relatively larger protein

7. Abscisic acid - e. A natural constraint that keeps developing embryos in their embryonic stage

8. DNA ladder - c. Acts as a reference that provides the information of the putative length of nucleotides

Laboratory 10

1. b. Embryo

Explanation: Embryo is the site where giberrellin is synthesized and released to endosperm via scutellum.

2. It inhibits the production of amylase.

Explanation: Amylase is the hydrolytic enzyme that converted starch to simple sugar. ABA inhibits this amylase production and remains the seed in dormant stage.

3. Barely seed as substrate, iodine solution for color development.

4. The wavelength used in spectrophotometer is 620 nm.