Question

TRUE OR FALSE:

1. Base-pairing rules apply from one DNA strand to its partner, but not along the sugar-phosphate "handrails" of a DNA strand.

2. DNA polymerase cannot copy point mutations, so they are not passed on from parent cells to daughter cells in cell division.

3. It is not possible for a human gene to work in any other organism.

4. Mutations in regulatory DNA sequences may be more important to evolution than mutations in genes.

5. The enzyme EcoRI cuts between the G and the A in the sequence GAATTC. The enzyme BamHI cuts between adjacent G nucleotides in the sequence GGATCC. The sticky ends that are generated by the two enzymes are complementary and will stick together.

Answer 1

1. True. The sugar phosphate groups bind to the next sugar phosphate group of the nucleotide. Base pairing rule doesn't apply to it. These sugar phosphate groups can bind to corresponding group of either purine or pyramidine.

2. False. DNA polymerase copies what ever is present. They can't differentiate between point mutation and native sequence.

3.false. human genes are successfully introduced into organisms like yeasts. Insulin gene is introduced into yeasts and insulin is prepared by this method.

4. False. Mutations in the genes contribute to evolution more than the mutation in the regulatory regions.

5. G AATTC ECor1 cut

G GATCC BamH1 cut. These strands are not complementary and they can't stick together. So it is False.