Question

Please explain as detailed as possible in several sentences. Thank you so much.

2. Please consider a scenario in which the phospholipids in the inner mitochondrial membrane (IMM) of all of a cell's mitochondria are changed so that about 20% of the protons in the intermembrane space (IMS) can leak into the matrix before they are transported by the ATP synthase. a) How will this affect the P/O ratio in this cell? b) How will this affect the respiratory quotient (RQ) for glucose in this cell? Please explain each answer.

No additional information necessary.

Answer 1

answer A

Before discussing the answer, need to etiscuss the chemiosmatic hypothesis, which eeplains Jenerahion ofATP. A1P is generated by phosphonyation of ADP. In kreb's cycle the reducing power is generated in the form of NADH and FADH, in the matrie of mitochondyia when protons foymed in the madrix are throun out of mitochondr ia A protonomohive Force is generated, these pro tons are teke inside the mito chondria thvolegh ATP synthase Enyme which phosphorylates ADP to generate ATP Now, it 201. of protons directy come to the matrix than through ATP Syn thase case a) P/o ratio is phosphate incorporated into ATP to Oxygen atom Teduced to uat er As HT ions ere. directy conming into the matrit of mito chondria, ATP synthase wit ach ess Theielore. ADP to ATP phosphorylaion wil reduce thereby reducina the Plo raho

answer b

case b) Kespiretony uotient of glucose is One, 1e The volume of carbon dioxide produced to the volune os Oygen consumed is equal The consunption Transport Chain depends on the availability of protons and i is independent of the ATP produced by ATP synthase (uhich in of Ox4gen in the Electron case a is decreasing because 20 ot Protons are directy coning inside the matrix Thesefore the Ra value of glucose will remain one and iwill not change of oxygen cons unption e tormation otwater by conbinahon of protons) is independent ot AS the Process and xygen Synthesis of ATP