Homework Answers
Being Hfr the donor E. coli strain and F- is the receptive strain. Therefore the genes of Hfr's genes would be the ones expressing in the recombinant colonies.
thr + : Can synthesize threonine
thr- : Can NOT synthesize threonine
leu - : Can NOT synthesize leucine
strr: resistant to streptomycin
strs: resistant to streptomycin (killed by streptomycin)
Recombinants would be able to grow in a medium without threonine and streptomycin, but with leucine.
Therefore the answer would be D) Minimal media + leucine
Recombination can take place, the question should indicate where between which genes are the sequences that allow recombination, but as it doesn't say anything you can assume that all the genes from Hfr (thr+ leu- strs) were inserted in the F-'s plasmid chromosome.
2. You have performed the following mating experiment using Hfr and F"strains of Escherichia coli:(4 marks)...
2. You have performed the following mating experiment using Hfr and F"strains of Escherichia coli:(4 marks) Hfr (asp+ trp+ mal+ StrepS) x F (asp-trp-mal- Strep). Strep = streptomycin, an antibiotic Asp = aspartic acid, an amino acid Trp = tryptophan, an amino acid Mal = maltose, a sugar molecule, can be used by some organisms as an energy source rather than glucose (a) What selective media would you use to score recombinant colonies that have received all of the markers...
2 What is the minimum genotype of a recombinant cell that grew on minimal media supplemented...
2 What is the minimum genotype of a recombinant cell that grew on minimal media supplemented with arginine, methionine and the antibiotic tetracycline but lacking the essential amino acid isoleucine 3 How would you determine if your recombinant cells had also acquired the tryptophan gene from the Hart Complete the following table indicating which of the organisms (F and/or Hfr used in this experiment would be expected to grow on the given media. Unless specified, MMD is just minimal medium...
A streptomycin-sensitive Hfr strain, prototrophic for threonine (thr), leucine (leu), arginine (arg), and histidine (his), was...
A streptomycin-sensitive Hfr strain, prototrophic for threonine
(thr), leucine (leu), arginine (arg), and histidine (his), was
allowed to conjugate with a streptomycin-resistant F- strain whose
genotype was thr- leu- arg- his-. When plated on complete medium
containing streptomycin, a number of colonies arose. Cells from
five of these colonies were then tested on minimal medium
supplemented with added nutrients as indicated below. What were the
genotypes of the five colonies. PLEASE EXPLAIN.
MINIMAL MEDIUM PLUS COLONIES THR LEU THR HIS...
Hi, I like to make sure I am staying on track with the thought process and...
Hi, I like to make sure I am staying on track with the thought
process and was curious if anyone can help with A-D. Thank you so
much for the help!
7. (12 pts) You have two Hfr strains of E. coli that carry the F plasmid integrated at different places in the chromosome. During conjugation, strain HfrA transfers the leucine biosynthetic operon only after the rest of the E. coli chromosome has been transferred. During conjugation, strain HfrB transfers...
BIS 101 Homework 04 Dr. Crowder nexperiment is performed in Escherichia coli using the parents Hfr...
BIS 101 Homework 04 Dr. Crowder nexperiment is performed in Escherichia coli using the parents Hfr arg str and Farg lys met his stor. The mating was interrupted at several d on several different media with the following results. The table below shows the number of colonies obtained on different media as es obtained on different media as indicated. a Minimal medium plus streptomycin plus Histidine Arginine Arginine Lysine Histidine Histidine Methionine Methionine Lysine ine Arginine Lysine Methionine Time (min.)...
Three autotrophic strains of E. coli (#1-3) of genotype a(-)b(+) are mixed in all possible combinations...
Three autotrophic strains of E. coli (#1-3) of genotype
a(-)b(+) are mixed in all possible combinations with three
auxotrophic strains (#4-6) of genotype a(+)b(-), and subsequently
plated on basal media.
A few recombinants form colonies when the following strains
are mixed: 2+4 and 3+6
Many recombinants form colonies when the following strains are
mixed: 1+6 and 2+5
All other combinations produce no surviving recombinants on
basal media.
What are the mating types (F+, F-, Hfr) of all six
strands?
Three...
3. (10 pts) A friend sets up the following cross between an Hfr strain and an...
3. (10 pts) A friend sets up the following cross between an Hfr strain and an F- strain; Hfr: rif lac str pheA X F:rif lac str pheA In the Hfr, the F factor is integrated at 9 minutes on the E. coli genetic map and transfers the genome in a counter clockwise direction, similar to CSHI19 Gene function Position on genetic map Gene Name RNA polymerase (target of rifamycin) lactose utilization phenylalanine biosynthesis ribosomal protein (target of streptomycin) rpoB...
4. You've run an interrupted mating conjugation experiment with Hfr cells that are hmm* omgpoot and...
4. You've run an interrupted mating conjugation experiment with Hfr cells that are hmm* omgpoot and streptomycin resistant (str). Your recipient cell is F-hmm omg poo' and streptomycin susceptible (str). Below are the results from that interrupted mating experiment. frequency of Hfr genetic characters among str-resistant exconjugants a) If you attempted to grow your exconjugants (those recipient cells that underwent conjugation) at 20 minutes on a complete media plate that contained streptomycin, would you expect to see growth? + X...
Question 10 View Policies Current Attempt In Progress In a conjugation experiment between two strains of...
Question 10 View Policies Current Attempt In Progress In a conjugation experiment between two strains of E.coli (streptomycin-sensitive Hfr strain and a streptomycin-resistant F-straint, aliquots were extracted from the mating mixture at various time points and plated on minimal media supplemented with streptomycin and a variety of amino acids. The number of conjugates at each time point on each type of media are shown in the table below. What can you conclude from these data!! Time (minutes) Medium MM (-)...
Conjugations are set up between E. coli of the genotypes indicated below. Bacteria are incubated for...
Conjugations are set up between E. coli of the genotypes indicated below. Bacteria are incubated for 10 minutes and then plated on minimal media with a concentration of azide (azi) sufficient to kill azi - sensitive E. coli . Predict how many Hfr, F+ and F - colonies (many, few, none) will appear on the azi plates and how they would come about. Note: the leu locus is very near the origin of transfer, whereas the azi locus is not....
2. You have performed the following mating experiment using Hfr and F"strains of Escherichia coli:(4 marks) Hfr (asp+ trp+ mal+ StrepS) x F (asp-trp-mal- Strep). Strep = streptomycin, an antibiotic Asp = aspartic acid, an amino acid Trp = tryptophan, an amino acid Mal = maltose, a sugar molecule, can be used by some organisms as an energy source rather than glucose (a) What selective media would you use to score recombinant colonies that have received all of the markers...
2 What is the minimum genotype of a recombinant cell that grew on minimal media supplemented with arginine, methionine and the antibiotic tetracycline but lacking the essential amino acid isoleucine 3 How would you determine if your recombinant cells had also acquired the tryptophan gene from the Hart Complete the following table indicating which of the organisms (F and/or Hfr used in this experiment would be expected to grow on the given media. Unless specified, MMD is just minimal medium...
A streptomycin-sensitive Hfr strain, prototrophic for threonine
(thr), leucine (leu), arginine (arg), and histidine (his), was
allowed to conjugate with a streptomycin-resistant F- strain whose
genotype was thr- leu- arg- his-. When plated on complete medium
containing streptomycin, a number of colonies arose. Cells from
five of these colonies were then tested on minimal medium
supplemented with added nutrients as indicated below. What were the
genotypes of the five colonies. PLEASE EXPLAIN.
MINIMAL MEDIUM PLUS COLONIES THR LEU THR HIS...
Hi, I like to make sure I am staying on track with the thought
process and was curious if anyone can help with A-D. Thank you so
much for the help!
7. (12 pts) You have two Hfr strains of E. coli that carry the F plasmid integrated at different places in the chromosome. During conjugation, strain HfrA transfers the leucine biosynthetic operon only after the rest of the E. coli chromosome has been transferred. During conjugation, strain HfrB transfers...
BIS 101 Homework 04 Dr. Crowder nexperiment is performed in Escherichia coli using the parents Hfr arg str and Farg lys met his stor. The mating was interrupted at several d on several different media with the following results. The table below shows the number of colonies obtained on different media as es obtained on different media as indicated. a Minimal medium plus streptomycin plus Histidine Arginine Arginine Lysine Histidine Histidine Methionine Methionine Lysine ine Arginine Lysine Methionine Time (min.)...
Three autotrophic strains of E. coli (#1-3) of genotype
a(-)b(+) are mixed in all possible combinations with three
auxotrophic strains (#4-6) of genotype a(+)b(-), and subsequently
plated on basal media.
A few recombinants form colonies when the following strains
are mixed: 2+4 and 3+6
Many recombinants form colonies when the following strains are
mixed: 1+6 and 2+5
All other combinations produce no surviving recombinants on
basal media.
What are the mating types (F+, F-, Hfr) of all six
strands?
Three...
3. (10 pts) A friend sets up the following cross between an Hfr strain and an F- strain; Hfr: rif lac str pheA X F:rif lac str pheA In the Hfr, the F factor is integrated at 9 minutes on the E. coli genetic map and transfers the genome in a counter clockwise direction, similar to CSHI19 Gene function Position on genetic map Gene Name RNA polymerase (target of rifamycin) lactose utilization phenylalanine biosynthesis ribosomal protein (target of streptomycin) rpoB...
4. You've run an interrupted mating conjugation experiment with Hfr cells that are hmm* omgpoot and streptomycin resistant (str). Your recipient cell is F-hmm omg poo' and streptomycin susceptible (str). Below are the results from that interrupted mating experiment. frequency of Hfr genetic characters among str-resistant exconjugants a) If you attempted to grow your exconjugants (those recipient cells that underwent conjugation) at 20 minutes on a complete media plate that contained streptomycin, would you expect to see growth? + X...
Question 10 View Policies Current Attempt In Progress In a conjugation experiment between two strains of E.coli (streptomycin-sensitive Hfr strain and a streptomycin-resistant F-straint, aliquots were extracted from the mating mixture at various time points and plated on minimal media supplemented with streptomycin and a variety of amino acids. The number of conjugates at each time point on each type of media are shown in the table below. What can you conclude from these data!! Time (minutes) Medium MM (-)...
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