Conjugations are set up between E. coli of the genotypes indicated below. Bacteria are incubated for...
Conjugations are set up between E. coli of the genotypes indicated below. Bacteria are incubated for 10 minutes and then plated on minimal media with a concentration of azide (azi) sufficient to kill azi - sensitive E. coli . Predict how many Hfr, F+ and F - colonies (many, few, none) will appear on the azi plates and how they would come about. Note: the leu locus is very near the origin of transfer, whereas the azi locus is not.
A) Hfr azis leu+ x F- azir leu-
B) Hfr azis leu- x F- azir leu+
C) Hfr azis leu+ x F+ azir leu-
D) F+ azis leu+ x F- azir leu-
Could you please explain the reasoning behind why there would be that many colonies, I already have the answers. Also, could you explain what it means that the locus is near the origin of transfer in regards to how many colonies there would be? THANK YOU
Homework Answers
Add Answer to:
Conjugations are set up between E. coli of the genotypes
indicated below. Bacteria are incubated for...
1) Genetics a) Which of the following genotypes have one haplotype in common with the genotype...
1) Genetics a) Which of the following genotypes have one haplotype in common with the genotype aBcdE/ABcDe? abdeE/ABcDE ABcDE/ABCDe abCDE/aBcdE ABcde/abcde b) Consider an experiment in which an Hfr strain of E. coli bacteria with the StrS, his+, met+ alleles in its genome is mixed with an F- strain of E. coli with the StrR, his-, and met- alleles in its genome. After the cells have been cultured together for 8 hours, the mixture of cells is plated on minimal...
3. (7) Suppose that you have selected E. coli that can grow only when the medium...
3. (7) Suppose that you have selected E. coli that can grow only
when the medium is supplemented with cysteine. You isolate one
mutant that you believe is defective in a new gene require for
cysteine biosynthesis - cysX .
You have two strains of bacteria:
Hfr cysX - gal+ his+ lac+ pur+ trp+ ton s
F - cysX+ gal - his - lac - pur - trp - ton R
A) What supplements would you need to add to...
2a. (4 pts) A potent mutagen was added to an E. coli culture growing in rich...
2a. (4 pts) A potent mutagen was added to an E. coli culture growing in rich broth. Prior experiments established that this F cell line, designated WWU113, could not synthesize leucine. You are interested in measuring the rate of induced reverse mutation. You plan to grow a liquid culture of the leu strain and then plate various dilutions of the culture onto selective media. What media should you use to grow up the liquid culture? This will be Media A...
2. You have performed the following mating experiment using Hfr and F"strains of Escherichia coli:(4 marks)...
2. You have performed the following mating experiment using Hfr and F"strains of Escherichia coli:(4 marks) Hfr (asp+ trp+ mal+ StrepS) x F (asp-trp-mal- Strep). Strep = streptomycin, an antibiotic Asp = aspartic acid, an amino acid Trp = tryptophan, an amino acid Mal = maltose, a sugar molecule, can be used by some organisms as an energy source rather than glucose (a) What selective media would you use to score recombinant colonies that have received all of the markers...
3. (10 pts) A friend sets up the following cross between an Hfr strain and an...
3. (10 pts) A friend sets up the following cross between an Hfr strain and an F- strain; Hfr: rif lac str pheA X F:rif lac str pheA In the Hfr, the F factor is integrated at 9 minutes on the E. coli genetic map and transfers the genome in a counter clockwise direction, similar to CSHI19 Gene function Position on genetic map Gene Name RNA polymerase (target of rifamycin) lactose utilization phenylalanine biosynthesis ribosomal protein (target of streptomycin) rpoB...
please answer question 4a-c? What is the recombination frequency between trp and cysX? E) What is...
please answer question 4a-c?
What is the recombination frequency between trp and cysX? E) What is the recombination frequency between ton and cysX? F) What is the order of the three genes? Indicate the distance between them. 4. (3) Suppose that you mate a lac+ Hfr strain with the genotype str 1+ O+ 2+ y + (that you grew in the absence of lactose) with a lac-F-strain with the genotype str - O+ Z-y! You allow sufficient time for the...
a) During the exponential phase of bacterial growth, the lab strain of E. coli has doubling...
a) During the exponential phase of bacterial growth, the lab strain of E. coli has doubling time of about 30 minutes. If you start with 10,000 cells per mL of culture, and let them grow exponentially 2 hours in a flask, what is the final density of the culture? Show and explain your calculations. b) If you plated a100μL of 1:1000 dilution that culture (after it had grown in a flask for 2 hrs) how many colonies would you expect...
A. You have been given a tube of E. coli. You are asked to make 1...
A. You have been given a tube of E. coli. You are asked to make 1 mL total volume of 10-1 dilution of the bacterial culture. Explain how you would do this. Show all necessary calculations. ____ ml cells + _____ ml water = 1 ml (total volume) B. Next, you were asked to make a 10-2 dilution of the bacterial sample. Explain how you would perform this. Show all necessary calculations. You have bacteria at a concentration of 2...
2 What is the minimum genotype of a recombinant cell that grew on minimal media supplemented...
2 What is the minimum genotype of a recombinant cell that grew on minimal media supplemented with arginine, methionine and the antibiotic tetracycline but lacking the essential amino acid isoleucine 3 How would you determine if your recombinant cells had also acquired the tryptophan gene from the Hart Complete the following table indicating which of the organisms (F and/or Hfr used in this experiment would be expected to grow on the given media. Unless specified, MMD is just minimal medium...
A generalized transducing phage is used to transfer genes from a thr(+)bio(+)thi(+) donor strain of E....
A generalized transducing phage is used to transfer genes from a thr(+)bio(+)thi(+) donor strain of E. coli to a thr(-)bio(-)thi(-) recipient strain. When transductants selected on a medium containing thiamine and threonine are tested for growth on other media, many more colonies are found on media supplemented only with threonine than on media supplemented only with thiamine. No transductants survive on minimal media. What is the correct order for these three genes? *I have seen multiple different answers for this,...
3. (7) Suppose that you have selected E. coli that can grow only
when the medium is supplemented with cysteine. You isolate one
mutant that you believe is defective in a new gene require for
cysteine biosynthesis - cysX .
You have two strains of bacteria:
Hfr cysX - gal+ his+ lac+ pur+ trp+ ton s
F - cysX+ gal - his - lac - pur - trp - ton R
A) What supplements would you need to add to...
2a. (4 pts) A potent mutagen was added to an E. coli culture growing in rich broth. Prior experiments established that this F cell line, designated WWU113, could not synthesize leucine. You are interested in measuring the rate of induced reverse mutation. You plan to grow a liquid culture of the leu strain and then plate various dilutions of the culture onto selective media. What media should you use to grow up the liquid culture? This will be Media A...
2. You have performed the following mating experiment using Hfr and F"strains of Escherichia coli:(4 marks) Hfr (asp+ trp+ mal+ StrepS) x F (asp-trp-mal- Strep). Strep = streptomycin, an antibiotic Asp = aspartic acid, an amino acid Trp = tryptophan, an amino acid Mal = maltose, a sugar molecule, can be used by some organisms as an energy source rather than glucose (a) What selective media would you use to score recombinant colonies that have received all of the markers...
3. (10 pts) A friend sets up the following cross between an Hfr strain and an F- strain; Hfr: rif lac str pheA X F:rif lac str pheA In the Hfr, the F factor is integrated at 9 minutes on the E. coli genetic map and transfers the genome in a counter clockwise direction, similar to CSHI19 Gene function Position on genetic map Gene Name RNA polymerase (target of rifamycin) lactose utilization phenylalanine biosynthesis ribosomal protein (target of streptomycin) rpoB...
please answer question 4a-c?
What is the recombination frequency between trp and cysX? E) What is the recombination frequency between ton and cysX? F) What is the order of the three genes? Indicate the distance between them. 4. (3) Suppose that you mate a lac+ Hfr strain with the genotype str 1+ O+ 2+ y + (that you grew in the absence of lactose) with a lac-F-strain with the genotype str - O+ Z-y! You allow sufficient time for the...
2 What is the minimum genotype of a recombinant cell that grew on minimal media supplemented with arginine, methionine and the antibiotic tetracycline but lacking the essential amino acid isoleucine 3 How would you determine if your recombinant cells had also acquired the tryptophan gene from the Hart Complete the following table indicating which of the organisms (F and/or Hfr used in this experiment would be expected to grow on the given media. Unless specified, MMD is just minimal medium...
Most questions answered within 3 hours.
-
A real object is located 20.6 cm from a diverg- ing lens of
focal length 38.5...
asked 28 minutes ago -
The primary difference between a breccia and a conglomerate is
that the conglomerate has more rounded...
asked 35 minutes ago -
A super-roach Bob is moving at a constant speed in a circular
path on the frying...
asked 35 minutes ago -
Market segments that can be effectively reached and served are
said to be ________.
measurable
profitable...
asked 18 minutes ago -
On January 1, 2021, the Allegheny Corporation purchased
equipment for $331,000. The estimated service life of...
asked 23 minutes ago -
At the local hockey rink, a puck with a mass of 0.12 kg is given
an...
asked 16 minutes ago -
For this lab, you will be writing search algorithms. I
have provided method signatures for you...
asked 18 minutes ago -
1. What form of energy do cells rely on most to carry out
important processes?
2....
asked 19 minutes ago -
The first-order reaction C4H8→2C2H4 has the reaction constant k
of 7.60×10−3 s−1. If the initial concentration...
asked 27 minutes ago -
TRUE OR FALSE
When an investor uses futures in a market timing strategy, the
only objective...
asked 30 minutes ago -
1..Research the microorganisms listed below. List the full
taxonomy (phylum, class, order, family, genus, species) of...
asked 31 minutes ago -
Suppose the gold spot price is $300/oz., the 1-year forward
price is $310.686, and the continuously...
asked 37 minutes ago