I got the last answer and it was wrong. Can you explain how you get your answer please?
Homework Answers
Correct answer is A. 3' TCGTCTTCAAGC
Because to analyze the gel you have to read the gel from the bottom to the top which will be the 5' to 3' direction of the daughter strand.
In this case the sequence of daughter strand is 5' AGCAGAAGTTCG 3'
So the original strand will be the reverse complementary of this sequence i.e. 3' TCGTCTTCAAGC 5'
N.B. You have to read the gel from the bottom to the top because the 1st nucleotide is the smallest hence it will appear last and the total DNA is largest so it will move least. So, you have to read the gel from the bottom.
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I got the last answer and it was wrong. Can you explain how you
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Pleaae answer this with explanation asap.I would really apprecuate it . 6. (4 points) Dideoxy sequencing...
Pleaae answer this with explanation asap.I would really apprecuate
it .
6. (4 points) Dideoxy sequencing of a fragment of DNA produces the gel pattern shown below, with the and+ to the left of the gel referring to the negative and positive electrodes of the gel, respectively. C T A G Which of the following statements about the gel products and the sequence of the DNA template are correct? The sequence of the template DNA is 5'-GACTCGACTGTACGTGC-3', te letter above...
9. (8 points) Use the figure below to answer the following questions about Sanger Dideoxy sequencing....
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got this wrong on my exam, can someone please explain how to get the answer? thank...
got this wrong on my exam, can someone please explain
how to get the answer? thank you
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I got 2/3 correct. Which one did I get wrong and please explain it to me?...
I got 2/3 correct. Which one did I get wrong and please explain
it to me? Thank you.
4. (6 points) Determine the hybridization of the indicated atoms. | hybridization of nitrogen 8p3 hybridization of carbon hybridization of nitrogen My OH E TTEN . .. ..11
based on the given graph how would these be answered? sa sequence from a sheep PCR...
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Pleaae answer this with explanation asap.I would really apprecuate
it .
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9. (8 points) Use the figure below to answer the following questions about Sanger Dideoxy sequencing. GATC 11.1 This figure represents a portion of a polyacrylamide gel used to separate DNA fragments produced from a Sanger dideoxy DNA sequencing reaction. Write the sequence of the boxed portion of the newly synthesized (nascent) strand (in the 5' to 3' direction). Label the 5' and 3' ends of the DNA sequence. 11 il Write the DNA sequence of the complementary template strand...
got this wrong on my exam, can someone please explain
how to get the answer? thank you
5 pts Is. Draw the products for the following reaction. Label then major ami minor + H2O miros
NEED HELP WITH THESE QUESTIONS. PLEASE ANSWER ALL AND EXPLAIN AS
WELL. THANKSSSSSSS
1. You want to clone a gene from a donor vector to a host vector. List the correct order of events in the process of cloning a. Perform ligation reaction of cloned gene and host vector. b. Perform double digestion of both donor and host vectors with the 2 restriction enzymes c. Examine donor and host vectors for restriction sites d. Purify cloned gene from donor vector...
Which of the following statements is correct concerning operon gene control? Positive control requires an activator protein to stimulate transcription of the structural genes within an operon. In negative control, a repressor protein inhibits or turns off transcription of the structural genes within the operon. An inducible operon normally is not transcribed. It requires an inducer molecule to stimulate transcription either by inactivating a repressor protein in a negative inducible operon or by stimulating the activator protein in a positive...
I got 2/3 correct. Which one did I get wrong and please explain
it to me? Thank you.
4. (6 points) Determine the hybridization of the indicated atoms. | hybridization of nitrogen 8p3 hybridization of carbon hybridization of nitrogen My OH E TTEN . .. ..11
based on the given graph how would these be answered?
sa sequence from a sheep PCR product. The template used was genomic DNA and the forward and se primers were designed to amplify partofthe priongene, which encodes the causative agentot scrapie. is black;Cisblue; Ais green: Tisred. The forward primerwasusedasasequencing primer. TheNattheten ow means that the computer can't decide which nucleotide is at that position in the sequence because there are two peaks (red=T and blue-C) that are the same height....
please help
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