The map below was constructed using information from an interrupted mating experiment. Str# refers to the...
An Hfr strain is used to map four genes in an interrupted mating experiment. The cross is Hfr/a+b+c+ rifs ×F−a−b−c− rifr . (No map order is implied in the listing of the alleles; rifr is resistance to the antibiotic rifampicin.) The a+gene is required for the biosynthesis of nutrient A, the b+ gene for nutrient B, c+ for nutrient C. The cross is initiated at time = 0, and at various times, the mating mixture is plated on medium. Each plate contains minimal medium (MM) plus rifampicin....
1. (4) The following are the results from an interrupted mating experiment. Your supervisor has asked you to interpret these results and draw a map showing the distances between the genes. The experimental set up was as follows: Hfr arg + mal + met + pur + pyr + xyl + azi s X F - arg- mal- met- pur- pyr- xyl- azir Exconjugants were plated on medium containing no arginine, maltose as the sole carbon source, and azide at...
1. (4) The following are the results from an interrupted mating experiment. Your supervisor has asked you to interpret these results and draw a map showing the distances between the genes. The experimental set up was as follows: Hfr arg + mal + met + pur + pyr + xyl + azi s X F - arg- mal- met- pur- pyr- xyl- azir Exconjugants were plated on medium containing no arginine, maltose as the sole carbon source, and azide at...
Sam is working in a genetics lab this summer in order to gain more hands-on experience before he applies to graduate school. The professor with whom he is working is particularly interested in studying a new strain of E.coli and assigns Sam an interrupted mating experiment to perform. Sam is attempting to determine the order of 7 different genes on the bacterial chromosome: Ger, Fra, Lod, Moc, Bla, Jat, and Arp. Sam used four different Hfr strains (Hfr 1, Hfr2,...
Genetics Question: I am confused on the second part of the question- determining the information for both the donor and recipient. Thank you! different interrupted mating experiments, you obtain the following data for 2. Ih three the time of entry of the following genes: arg, met, leu, ser, gal, val, and phe. All of these experiments were conducted using Hfr strains (Hfr 1, Hfr 2, and Hfr3) derived from the same original strain of E. coli. Experiment 1 (Hfr 1)...