Question

What were the principal technical limitations of using electron microscopes that had to be overcome and how were they addressed to make modern cryo-EM possible?

Answer 1

d. What were the principal technical limitath of urring 4M that had to be overcome & how were the adressed to make подап сочь ем роті [4) Ans: The electron microscope is a practical application of de Broglie wars The basic principal on which the electron microscope wooks is - la the election behares like light rays having short wavelength 16 an electron beam can be focussed by suitable electric er magnetic field (u) Electrons are negatively charged particles... (i) since electrons are no easily absorbed in air at atmospheric presswa, the EM has to operate in vacuum. 100.000 r. I OKV 2100, o 12.27.xlo-10m 0.04 Å LOXLOVTO h this voltage will correspond to andare length of about 0.03884 or 0.04 å opprou), in Electron microscope makes use of the ware properties of the moving electron. V 2 Cryogenic electron microscopy (cryo-EM) enables structure determination of macromolecular objects & their assemblies. Although the techniques have been developing for nearly four decades, they have gained widespread attention in recent years due to technical advances on numerous fronts, enabling traditional microscopists to break into the world of molecular structural biology. Many semples cas now be routinely analyzed at near- atomie resolution using standard imaging and image analysis techniques, However, minerous challenges to conventioned workflows remoun, & continued technical advances open entirely novel opportunities for discovery & exploration. Here, I will review some of the main methods sinfiound oryo - EM with as. emphasis specifically on single particle analysis, & I will highlight challenges, open questions & opportunities for methodology development. comment in the comment box if any doubt please Thanks! with can Cs