You have a standard stock containing (1.67 mM glucose + 1.67 mM fructose). Calculate the glucose...
live spectrophe Using test tubes, Proceed with the determination as shown in the table below to Reagents (ml) Sample Blank Std Sto2 Std Glucose stock (1.5 mg/mL) 0.1 0.2 0.4 water I 2.4 2.3 2.1 Sample 2.5 (Unknown) DNS reagent 1.0 1.0 1.0 1.0 1.0 2.5 1. Shake the tubes, put all at the same time in boiling water bath (must be boiling for 5 minutes, 2. After heating, cool the tubes in cold water and then add 6.5 ml...
You have a stock solution that contains 24 mg/ml of protein. You want to make a set of standards with the following concentrations: 12 mg/ml, 6 mg/ml, 3 mg/ml and 1.5 mg/ml. You need a minimum of 500 ul of each standard after all dilutions are completed. Using the technique of serial dilution, explain how you would make your standards by filling in the chart below. Std 1 Std 2 Std 3 Std 4 concentration (mg/ml) 12 6 3...
Table 1; of absorbance vs. concentration on a graph sheet (use pencil, properly amylase from two sources as samples (unknowns) were also treated in d. The experimental set-up of Table 1, indicate whether it was derived using serial e. In Table 2, considering the original salivary amylase samples, which one of the b. Plot curve labeled graph will be noted or generate an excel-sheet): Use graph to extrapo late the unknowns and determine their concentrations; e. Salivary the same protocol...
Experiment # Starting Substrate Concentration Actual Substrate Concentration Substrate Added (ml) Buffer Added (ml) 1.5 mm 1.5 1.0 mm Nm 1.0 0.5 mm 0.50 0.250 mm 0.250 0.125 mm 0.120 1.38 0.060 MM 0.060 1.44 0.030 mm 0.030 1.47 0.015 mm 0.015 1.485 *Actual substrate concentration is calculated by taking the starting substrate concentration into the final volume of each reaction after enzyme has been added. Note that the total volume for each reaction prior to the addition of enzyme...
29. You have the following concentrated commercial reagents on hand; make 1 L of a 6 N reagent from each of them. What is the molarity of each concentrated reagent? How to make it? NFLOH sp. gr. = 0.90; 58% assay (V=1) H(CH3C00) sp. gr. = 1.05; 99% assay (v-1) 30. How much 0.02 N HSO, can you make from 200 mL of 4N HSO.? 31. How much Cuso, 8H20 will you need to weigh out to substitute for 120...
Lab 5: Additional Dilution Problems You have a stock solution that contains 24 mg/ml of protein. You want to make a set of standards with the following concentrations: 12 mg/ml, 6 mg/ml, 3 mg/ml and 1.5 mg/ml You need a minimum of 500 ul of each standard after all dilutions are completed. Using the technique of serial dilution, explain how you would make your standards by filling in the chart below. Std 4 Std 1 12 Std 2 6 Std...
help please! thank you! 2. In the Spectrophotometric analysis of your unknown compound you will create a standard curve with a stock solution of 0.400 M Cu. Determine the concentration of Cu in tubes 1 -4. Complete the values here and in this same table in your lab book. tube Contents Concentration of Cu ions (M Approximately 10 mL of1 M HNO 4.0 mL of 0.400 M Cu (stock standard) and 6.0 mL 1 M HNO 2 7.0 mL of...
4) You have been given the job of designing an experiment to test the effects of an inhibitor on the coronavirus polymerase. Assume you have a stock solution containing purified polymerase protein. You want to know how much polymerase protein you have in the stock solution. To do this, you will use the Bradford Assay. If you mix 50L of polymerase protein stock solution with 100L of water, perform the Bradford Assays and get a resulting OD 595mm of 0.6,...
please hlep me answer those three questions asap. Please ignore question 7. Question 7 Working with buffers You need 100 ml of 120 mM phosphate buffer and you have two stock solutions from which you can make the buffer; 0.6 M NaH2PO4 (the acid form) and 0.6 M Na2HP04 (the base form). You need to add 3 times more of the base form than the acid form to achieve the desired pH. How will you make this solution? Question 8...
Electrolytes, Concentration and Dilution of Solutions n this part of the experiment, you will make a dilution of a stock solution of known concentration and calculate the concentration of the dilute solution. DILUTION OF A STOCK SOLUTION PART C: Measurements and Observations Procedure Check out a 100-mL volumetric flask from your instructor. Fill test tube about 2/3 concentration of the active ingredient from its label including units. Record 1. orlt O " concentration of active ingredient. full of "Magic 2....