Question

live spectrophe Using test tubes, Proceed with the determination as shown in the table below to Reagents (ml) Sample Blank Std Sto2 Std Glucose stock (1.5 mg/mL) 0.1 0.2 0.4 water I 2.4 2.3 2.1 Sample 2.5 (Unknown) DNS reagent 1.0 1.0 1.0 1.0 1.0 2.5 1. Shake the tubes, put all at the same time in boiling water bath (must be boiling for 5 minutes, 2. After heating, cool the tubes in cold water and then add 6.5 ml of distilled water to each tube. 3. By using a spectrophotometer, measure the absorbance (at 540 nm) of the samples and standards using a blank as a reference ADRO Fill the missing.parts in the following table Initial conc. Initial Vol. (mg/mL) (ml) Final vol. (mL) Final conc. (mgml) Absorbance 0.1 Std 1 02 Std 2 0.3 Std 3 0.25 ample known) MacBook Pro

Answer 1

	Initial conc.(mg/ml)	Initial vol (ml)	final vol. (ml)	Final conc.(mg/ml)	Absorbance
Std 1	1.5	0.1+2.4+1.0= 3.5	3.5+6.5 = 10.0	1.5×0.1/10.0 = 0.015	0.1
Std 2	1.5	0.2+2.3+1.0= 3.5	3.5+6.5 = 10.0	1.5×0.2/10.0 = 0.030	0.2
Std 3	1.5	0.4+2.1+1.0= 3.5	3.5+6.5= 10.0	1.5×0.4/10.0 = 0.060	0.3
Sample (unknown)	C1	2.5+1.0 = 3.5	3.5+6.5 = 10.0	C2	0.25