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Pre-lecture lesson 2.12: Linkage and Gene Mapping You are charged with testing whether 2 different genes are independently assorting during meiosis in Drosophila melanogaster, the fruit fly The 2 genes you will be observing for this experiment are: Black: a recessive allele of this gene (b) results in a dark body color (somewhat similar to ebony, but a richer dark coloration that is less ashen-colored). The dominant allele for this gone (B) produces wild type body color Vestigial: a recessive allele of this gene (vg) results in shriveled up wings-wings do develop as opposed to apterous, but they are deformed. The dominant allele for this gene (VG) produces a normal wing shape. We again produce a dihybrid individual by crossing 2 flies with genotypes: BB, vg vg X bb, VG VG so that the F1 individual from this cross will have genotype: B b, VG vg Our hypothesis is the same as it was before, but now with respect to these 2, new genes: The alleles of the genes Black and Vestigial are independently assorting during meiosis Below, explain how you can test this hypothesis using a genetic cross, and give your predictions for this hypothesis for the frequency with which you expect to find parental versus recombinant types among the offspring of your cross, IF our hypothesis of independent assortment is correct Drosophila melanogaster, the fruit fly Your answer
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Ans- F1 Individuals are crossed as follows:

BbVGvg X BbVGvg

F2 progeny-

9 - Wild type body colour with normal wings - 56 individual

3- Dark body colour with normal wings- 19 individual

3- wild type body colour with vestigial wings- 19 individual

1- Dark body colour with vestigial wings- 6 individual

If the progeny results in this ratio then the genes are assorting independently.

Futher to test the hypothesis we study a progeny of 100 flies in F2 generation with the cross shown above and then calculate the frequency of recombination among them.

If we get no.of individuals as listed above and when we calculate recombination frequency based on this data we'll get more than 50% recombinant ion, this clearly shows that genes are assorting independently and are not linked.

Recombination frequencies between 1-50% signifies that the two genes under consideration are 1-50CM apart and are linked or present on the same chromosome.

If recombination frequency are more than 50% then genes are not linked and are segregating independently.

If we will get more than 50% recombinants then the genes are assorting independently

and the resulting progeny has the population in the ratio of

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