Glucose-1,6-biphosphate is a metabolic regulator. Predict its effect on (a) hexokinase and (b) PFK
Glucose-1,6-biphosphate is a metabolic regulator. Predict its effect on (a) hexokinase and (b) PFK
Explain in detail how PFK-1, hexokinase, glucokinase, pyruvate kinase, glucose-6-phosphatase, fructose-1,6-bisphosphatase-1, pyruvate carboxylase & PEPCK are regulated & compare their regulation in liver vs. skeletal muscle & brain when blood glucose levels are high (i.e. a fed state) & low (i.e. a fasting or starvation state).
MATCH THE FOLLOWING a. PFK-1 b. FBpase-1 c. PFK-2 d. FBpase-2 e.cAMP- dependent protein kinase (PKA) WITH 1. makes F-2,6 -BP(fructose-2,6-biphosphate) 2. Activated by F-2,6-BP(fructose-2,6-biphosphate) 3. Activates the enzyme that breaks down F-2,6-BP 4. Inhibited by F-2,6-BP 5. Breaks down F-2,6-BP
GLYCOLYSIS Glucose-8 phosphate + Glucose 2 Fructose-6-phosphate 3 Fructose-1,6-biphosphate Dihydroxy-acetone-phosphate 5 Glyceraldehyde-3-phosphate VT6 2 (1,3-biphosphoglycerate) 7 3-phosphoglycerate 2-phosphoglycerate 9 phosphoenolpyruvate 10 Lactate Pyruvate Which two reactions of glycolysis requires an investment of ATP energy, and which enzyme catalyzes each reaction? Reaction Number Enzyme Reaction Number Enzyme Which two reactions of glycolysis generate energy in the form of ATP, and which enzyme catalyzes each reaction? Reaction Number Enzyme Reaction Number Enzyme Which glycolytic reaction generates energy in the form of NADH,...
1. Which of the folowing statements about glucokinase and hexokinase (that catalyze the same reaction) is TRUE? A. Glucokinase has a higher capacity than hexokinase to convert glucose to glucose 6 phosphate B. Hexokinase is only active in the liver where it can utilize multiple hexoses C. While glucokinase requires ATP for its action, hexokinase requires GTP D. Glucokinase can act on multiple glucose-like sugars (such as galactose) E. Hexokinase has a higher Km and easily handles all the glucose...
A metabolic intermediate of gluconeogenesis not found in glycolysis is: A. fructose 2.6-bisphosphate B. oxaloacetate C. phosphoenopyruvate D. fructose 1,6-bisphosphate in humans, gluconeogenesis: A can result in the conversion of amino acids to glucose, B. is essential in the conversion of fatty acids to glucose. OC. is activated by insulin, D. helps to reduce blood glucose concentration after a carbohydrate rich meal.
The first step in the metabolic breakdown of glucose is its phosphorylation to G6P: glucose(aq) + ATPlaq)→G6P(aq) + ADP(aq) + H+ (aq) The standard reaction Gibbs energy for the reaction is AG -34 kJ/mol at 37°C ▼ Part A Calculate the biological standard reaction Glbbs energy for the reaction at 37 C AG68.0 kJ/mol Submit Previous Answers Request Answer X Incorrect; Try Again; 2 attempts remaining Part B Calculate the equilibrium constant of the reaction in the thermodynamic standard (Kea)...
Concentrations of some glycolysis intermediates in the red blood cell (mM): Glucose Glucose-6-P Fructose-6-P Fructose-1,6-BP 1,3-bisphosphoglycerate 0.001 3-phosphoglycerate 5.0 0.083 0.014 0.031 2- phosphoglycerate Phosphoenolpyruvate Pyruvate ATP ADP Pi 0.030 0.023 0.051 1.85 0.14 1.0 0.12 3. Isotopic labeling (incorporating a rare isotope, eg. the radioactive 1C, into a molecule at a defined position) is an important technique for studying metabolism as it enables us to follow individual atoms through a metabolic pathway 2 a. If 4CO2 were bubbled through...
what is the effect of adding glucose in a mideum? does it affect its sensitivity or specificity? explain.
The first step in the metabolic breakdown of glucose is its phosphorylation to G6P: glucose(aq)+ATP(aq)→G6P(aq)+ADP(aq)+H+(aq)glucose(aq)+ATP(aq)→G6P(aq)+ADP(aq)+H+(aq) The standard reaction Gibbs energy for the reaction isΔGoΔGo= -34 kJ/mol at 37°C A. Calculate the biological standard reaction GIbbs energy for the reaction at 37°C. B. Calculate the equilibrium constant of the reaction in the thermodynamic standard (KeqKeq) at 37°C. Use 2 significant figures. c, Calculate the equilibrium constant of the reaction in the biological standard (K′eqKeq′) at 37°C. Use 2 significant figures.
1. Normally for an organism to increase its metabolic rate it must. a.) maintain a higher concentration of H+ (protons) in the cytoplasm of it cells then in its mitochondria. b.) pump more glucose into the mitochondria c.) pump more glucose out of the mitochondria d.) maintain a lower concentration of CO2 between the mitochondrial membranes e.) pump H+ (protons) across the inner mitochondrial membrane at a faster rate