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ng Learning The following table shows where different restriction endonu abbreviation R represents the purines (adenine...
The table shows where different restriction endonucleases (restriction enzymes) cleave DNA. The abbreviation R represents the...
The table shows where different restriction endonucleases (restriction enzymes) cleave DNA. The abbreviation R represents the purines (adenine and guanine). The pyrimidines (cytosine, thymine, and uracil) are abbreviated as Y. The abbreviation W represents adenine or thymine. Enzyme EcoRI EcoRV Target sequence 5' GAATTC 3 3' CTTAAG 5 5' GATATC 3 3' CTATAG 5 5' GGCC 3' 3' CCGG 5 5' AAGCTT 3 3' TTCGAA 5 5' RGGWCCY 3 3' YCCWGGR 5 Cleavage 5G AATTC 3' 3' CTTAA G 5'...
Need Part C answered only. The table shows where different restriction endonucleases (restriction enzymes) cleave DNA....
Need Part C answered only.
The table shows where different restriction endonucleases (restriction enzymes) cleave DNA. The abbreviation R represents the purines (adenine and guanine). The pyrimidines (cytosine, thymine, and uracil) are abbreviated as Y. The abbreviation W represents adenine or thymine. Enzyme EcoRI EcoRV Target sequence 5' GAATTC 3' 3' CTTAAG 5 5' GATATC 3 3' CTATAG 5 5' GGCC 3 3' CCGG 5 5' AAGCTT 3' 3' TTCGAA 5 5'RGGWCCY 3 3' YCCWGGR 5 Cleavage 5'G AATTC 3'...
Which of the following restriction endonucleases produces 3' overhangs? (position where the enzyme cuts is indicated...
Which of the following restriction endonucleases produces 3' overhangs? (position where the enzyme cuts is indicated by an arrow) A. EcoRI (5'-G↓AATTC-3') B. HindIII (5'-A↓AGCTT-3') C. EcoRV (5'-GAT↓ATC-3') D. PstI (5'-CTGCA↓G-3')
Identify two restriction endonucleases that could be used to make sticky ends near the 5’ end of this DNA sequence (uppe...
Identify two restriction
endonucleases that could be used to make sticky ends near the
5’ end of this DNA sequence (upper strand) so that
it could be incorporated into a new plasmid. You have a short list
of them in Table 9-2, and the specific, short sequences of bases
that other enzymes cut at are easily obtained from web resources.
You must cut as near to the 5' end as possible. Indicate the
specific sequences of bases for each endonuclease...
The table shows where different restriction endonucleases (restriction enzymes) cleave DNA. The abbreviation R represents the purines (adenine and guanine). The pyrimidines (cytosine, thymine, and uracil) are abbreviated as Y. The abbreviation W represents adenine or thymine. Enzyme EcoRI EcoRV Target sequence 5' GAATTC 3 3' CTTAAG 5 5' GATATC 3 3' CTATAG 5 5' GGCC 3' 3' CCGG 5 5' AAGCTT 3 3' TTCGAA 5 5' RGGWCCY 3 3' YCCWGGR 5 Cleavage 5G AATTC 3' 3' CTTAA G 5'...
Need Part C answered only.
The table shows where different restriction endonucleases (restriction enzymes) cleave DNA. The abbreviation R represents the purines (adenine and guanine). The pyrimidines (cytosine, thymine, and uracil) are abbreviated as Y. The abbreviation W represents adenine or thymine. Enzyme EcoRI EcoRV Target sequence 5' GAATTC 3' 3' CTTAAG 5 5' GATATC 3 3' CTATAG 5 5' GGCC 3 3' CCGG 5 5' AAGCTT 3' 3' TTCGAA 5 5'RGGWCCY 3 3' YCCWGGR 5 Cleavage 5'G AATTC 3'...
Identify two restriction
endonucleases that could be used to make sticky ends near the
5’ end of this DNA sequence (upper strand) so that
it could be incorporated into a new plasmid. You have a short list
of them in Table 9-2, and the specific, short sequences of bases
that other enzymes cut at are easily obtained from web resources.
You must cut as near to the 5' end as possible. Indicate the
specific sequences of bases for each endonuclease...
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