Which of the following restriction endonucleases produces 3' overhangs? (position where the enzyme cuts is indicated...
Which of the following restriction endonucleases produces 3' overhangs? (position where the enzyme cuts is indicated by an arrow)
A. EcoRI (5'-G↓AATTC-3')
B. HindIII (5'-A↓AGCTT-3')
C. EcoRV (5'-GAT↓ATC-3')
D. PstI (5'-CTGCA↓G-3')
Homework Answers
Option d is correct. Pstl will produce 3' overhangs.
The cutting will be like this 5' CTGCA 3' and 5' G 3'
Add Answer to:
Which of the following restriction endonucleases produces 3'
overhangs? (position where the enzyme cuts is indicated...
The table shows where different restriction endonucleases (restriction enzymes) cleave DNA. The abbreviation R represents the...
The table shows where different restriction endonucleases (restriction enzymes) cleave DNA. The abbreviation R represents the purines (adenine and guanine). The pyrimidines (cytosine, thymine, and uracil) are abbreviated as Y. The abbreviation W represents adenine or thymine. Enzyme EcoRI EcoRV Target sequence 5' GAATTC 3 3' CTTAAG 5 5' GATATC 3 3' CTATAG 5 5' GGCC 3' 3' CCGG 5 5' AAGCTT 3 3' TTCGAA 5 5' RGGWCCY 3 3' YCCWGGR 5 Cleavage 5G AATTC 3' 3' CTTAA G 5'...
Need Part C answered only. The table shows where different restriction endonucleases (restriction enzymes) cleave DNA....
Need Part C answered only.
The table shows where different restriction endonucleases (restriction enzymes) cleave DNA. The abbreviation R represents the purines (adenine and guanine). The pyrimidines (cytosine, thymine, and uracil) are abbreviated as Y. The abbreviation W represents adenine or thymine. Enzyme EcoRI EcoRV Target sequence 5' GAATTC 3' 3' CTTAAG 5 5' GATATC 3 3' CTATAG 5 5' GGCC 3 3' CCGG 5 5' AAGCTT 3' 3' TTCGAA 5 5'RGGWCCY 3 3' YCCWGGR 5 Cleavage 5'G AATTC 3'...
ng Learning The following table shows where different restriction endonu abbreviation R represents the purines (adenine...
ng Learning The following table shows where different restriction endonu abbreviation R represents the purines (adenine and guanine). The pyrimidines (cytosine, thymine, and uracil) are abbreviated as Y. The abbreviation W represents adenine or thymine cleases (restriction enzymes) cleave DNA. The Cleavage 5' G EcoRI 5 GAATTC 3 AATTC 3 5' 3 CTTAAG 5' EcoRV 5 GATATC 3 3' CTATAG 5 3' CTTAA 5 GAT ATC 3 Hae? Hindlll PpuMI5' RGGWCCY 3 3' CTA TAG 5 ?'CC GG5. 5 A...
Which of the following restriction enzymes do(es) generate sticky ends? A) Enzyme Recognition &n
Which of the following restriction enzymes do(es) generate sticky ends? A) Enzyme Recognition BamHI G↓GATCC CCTAG↑G B) Enzyme Recognition EcoRI G↓AATTC CTTAA↑G C) Enzyme Recognition HaeIII GG↓CC CC↑GG D) Enzyme Recognition HindIII A↓AGCTT TTCGA↑A E) all of the above, except c
7. Decide whether the DNA fragment shown below will be cut by the following restriction endonucleases:...
7. Decide whether the DNA fragment shown below will be cut by the following restriction endonucleases: EcoRI (5’-GAATTC-3’), AluI (5’-AGCT-3’), PstI (5’-CTGCAG-3’). For each one that cuts, how many products will be produced? 5’-AAGAATTGCGGAATTCGAGCTTAAGGGCCGCGCCGAAGCTTTAAA-3’ 3’-TTCTTAACGCCTTAAGCTCGAATTCCCGGCGCGGCTTCGAAATTT-5’
Identify two restriction endonucleases that could be used to make sticky ends near the 5’ end of this DNA sequence (uppe...
Identify two restriction
endonucleases that could be used to make sticky ends near the
5’ end of this DNA sequence (upper strand) so that
it could be incorporated into a new plasmid. You have a short list
of them in Table 9-2, and the specific, short sequences of bases
that other enzymes cut at are easily obtained from web resources.
You must cut as near to the 5' end as possible. Indicate the
specific sequences of bases for each endonuclease...
please answer question numbe 2. Restriction endonucleases are bacterial enzymes that recognize, bind, and cut DNA...
please answer question numbe 2.
Restriction endonucleases are bacterial enzymes that recognize, bind, and cut DNA strands at specific recognition sequences. They evolved to protect bacteria from bacterial viruses and are very useful for a wide variety of molecular biology applications. Some of the more common ones include EcoRI which recognizes the 6 bp sequence 5 'GAATTC 3' and cleaves the phosphodiester backbone after the G. Hind III_(AAGCTT), BamHI (G|GATCC), Pstl (CTGCAG), Not! (GC|GGCCGC), Ndel (CATATG) Kpnl (GGTAC^C), Bgl II...
Question 26 Refer to the image below. Which of the plate quadrants is the most useful...
Question 26 Refer to the image below. Which of the plate quadrants is the most useful for isolation of a single colony? Not yet answered Points out of 1.00 P Flag question 1 2 4 3 Select one: a 1 b. 2 Oc.3 O d. 4 Question 27 Which of the following is an end product of fermentation? Not yet answered Points out of 1.00 Select one: a glucose O b. carbon dioxide P Flag question C. oxygen d. fat...
A linear piece of DNA has the following restrictions sites: Xbal Noti Psti EcoRi 2 kb...
A linear piece of DNA has the following restrictions sites: Xbal Noti Psti EcoRi 2 kb 4 kb -> 1 kb 2 kb 3 kb You decided to set up an experiment where you added one of these restriction enzymes to this DNA. After this DNA was digested with that restriction enzyme, you separated the resulting fragment(s) using agarose electrophoresis. After the gel was stained with ethidium bromide, you observed the following gel (the DNA ladder is your reference standard...
Which of the following may represent one strand of a typical restriction enzyme recognition site? (CHOOSE...
Which of the following may represent one strand of a typical restriction enzyme recognition site? (CHOOSE ONE ANSWER BELOW) A. 5’ GACGAC 3’ B. 5’ GACCAG 3’ C. 5’ GAGCTG 3’ D. 5’ GACGTC 3’
The table shows where different restriction endonucleases (restriction enzymes) cleave DNA. The abbreviation R represents the purines (adenine and guanine). The pyrimidines (cytosine, thymine, and uracil) are abbreviated as Y. The abbreviation W represents adenine or thymine. Enzyme EcoRI EcoRV Target sequence 5' GAATTC 3 3' CTTAAG 5 5' GATATC 3 3' CTATAG 5 5' GGCC 3' 3' CCGG 5 5' AAGCTT 3 3' TTCGAA 5 5' RGGWCCY 3 3' YCCWGGR 5 Cleavage 5G AATTC 3' 3' CTTAA G 5'...
Need Part C answered only.
The table shows where different restriction endonucleases (restriction enzymes) cleave DNA. The abbreviation R represents the purines (adenine and guanine). The pyrimidines (cytosine, thymine, and uracil) are abbreviated as Y. The abbreviation W represents adenine or thymine. Enzyme EcoRI EcoRV Target sequence 5' GAATTC 3' 3' CTTAAG 5 5' GATATC 3 3' CTATAG 5 5' GGCC 3 3' CCGG 5 5' AAGCTT 3' 3' TTCGAA 5 5'RGGWCCY 3 3' YCCWGGR 5 Cleavage 5'G AATTC 3'...
ng Learning The following table shows where different restriction endonu abbreviation R represents the purines (adenine and guanine). The pyrimidines (cytosine, thymine, and uracil) are abbreviated as Y. The abbreviation W represents adenine or thymine cleases (restriction enzymes) cleave DNA. The Cleavage 5' G EcoRI 5 GAATTC 3 AATTC 3 5' 3 CTTAAG 5' EcoRV 5 GATATC 3 3' CTATAG 5 3' CTTAA 5 GAT ATC 3 Hae? Hindlll PpuMI5' RGGWCCY 3 3' CTA TAG 5 ?'CC GG5. 5 A...
Identify two restriction
endonucleases that could be used to make sticky ends near the
5’ end of this DNA sequence (upper strand) so that
it could be incorporated into a new plasmid. You have a short list
of them in Table 9-2, and the specific, short sequences of bases
that other enzymes cut at are easily obtained from web resources.
You must cut as near to the 5' end as possible. Indicate the
specific sequences of bases for each endonuclease...
please answer question numbe 2.
Restriction endonucleases are bacterial enzymes that recognize, bind, and cut DNA strands at specific recognition sequences. They evolved to protect bacteria from bacterial viruses and are very useful for a wide variety of molecular biology applications. Some of the more common ones include EcoRI which recognizes the 6 bp sequence 5 'GAATTC 3' and cleaves the phosphodiester backbone after the G. Hind III_(AAGCTT), BamHI (G|GATCC), Pstl (CTGCAG), Not! (GC|GGCCGC), Ndel (CATATG) Kpnl (GGTAC^C), Bgl II...
Question 26 Refer to the image below. Which of the plate quadrants is the most useful for isolation of a single colony? Not yet answered Points out of 1.00 P Flag question 1 2 4 3 Select one: a 1 b. 2 Oc.3 O d. 4 Question 27 Which of the following is an end product of fermentation? Not yet answered Points out of 1.00 Select one: a glucose O b. carbon dioxide P Flag question C. oxygen d. fat...
A linear piece of DNA has the following restrictions sites: Xbal Noti Psti EcoRi 2 kb 4 kb -> 1 kb 2 kb 3 kb You decided to set up an experiment where you added one of these restriction enzymes to this DNA. After this DNA was digested with that restriction enzyme, you separated the resulting fragment(s) using agarose electrophoresis. After the gel was stained with ethidium bromide, you observed the following gel (the DNA ladder is your reference standard...
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