Which of the following may represent one strand of a typical restriction enzyme recognition site? (CHOOSE ONE ANSWER BELOW)
5’ GACGAC 3’
5’ GACCAG 3’
5’ GAGCTG 3’
5’ GACGTC 3’
D) 5' GACGTC 3'
It is the best recognition site
Restriction enzyme cleaves the DNA into fragments at a specific site which is known as recogniton site.
These will be specific for each enzyme basis on their classes.
Which of the following may represent one strand of a typical restriction enzyme recognition site? (CHOOSE...
The recognition sequence for the restriction enzyme BamHI is '5-GGATCC-3' on one strand. What would be the recognition site on the complementary strand?
1.When cloning a PCR product into a plasmid using restriction enzymes, the restriction enzyme recognition sequences in the PCR product most likely came from _______, and the restriction enzyme recognition sequences in the plasmid most likely came from ________. a. A multiple cloning site / the primers b. The primers / a multiple cloning site c. Both came from primers d. Both came from the multiple cloning site e. Naturally present in the gene of interest / the multiple cloning...
The first three bases of the 6-base recognition cleavage site of the restriction enzyme, HindIII are AAG. What is the complete sequence of this 6 bp site? Provide an explanation for your answer.
Which of the following restriction enzymes do(es) generate sticky ends? A) Enzyme Recognition BamHI G↓GATCC CCTAG↑G B) Enzyme Recognition EcoRI G↓AATTC CTTAA↑G C) Enzyme Recognition HaeIII GG↓CC CC↑GG D) Enzyme Recognition HindIII A↓AGCTT TTCGA↑A E) all of the above, except c
If you cut the following single stranded DNA fragment with a restriction enzyme with restriction site of 5’GAATTC 3” and the cutting point between G and A. a. How many fragments you will get b. Specify the size (Number of bases) and the sequence of each fragment, pay attention to DNA direction (5’-3’) 5” ACATTGTCCGGGAATTC CGGGCTAGGCAT T GAATTGGAACA GAATTC GGGCCCGATCCGTA 3
1. The full-length SNFI gene was inserted into pLexA at the BamHI restriction site to create pLex-Snfl. To do this, the BamHI recognition sequence was introduced onto both the 5' and 3' ends of the SNFI DNA. Add the necessary nucleotides onto both strands of the DNA represented below: 3 SNF1 sense strand SNF1 antisense strand 5 3' Show the sequence that will result from BamHI digestion: 3 5' SNF1 sense strand SNF1 antisense strand 5" 1. The full-length SNFI...
how many times should a restriction enzyme with a recognition of TCGA cut a DNA sequence of 1000 bases? a) 2 b)4 c) 8 d) 16 e) 32
2. 3. Give the recognition sequence for the restriction enzyme Eael in a dsDNA sequence, indicate the cleavage sites. (2) With reference to your PCR amplicon, detail the terminal DNA sequences at the two ends of the fragment that will be generated after digestion with Eael (indicate these in the two blocks below). What type of ends are generated by this enzyme (blunt/sticky, 5' or 3' overhang)? PCR amplicon بیا بیا
Which of the following mutations in the active site of the enzyme enolase is most likely to result in an enzyme that still catalyzes its reaction? Choose one: A. Glu295→Lys295 B. Glu295→Asp295 C. Lys396→Ile396 D. Lys396→Glu396.
he full-length SNFI gene was inserted into pLexA at the BamHl restriction site to create pl.ex-Snf. To do this, the BamHI recognition sequence was introduced onto both the 5 and 3 ends of the SNFI DNA. Add the necessary nucleotides onto both strands of the DNA represented below: 5' 3 SNF1 sense strand SNF1 antisense strand 3 5 Show the sequence that will result from BamHl digestion: 5" 3 SNF1 sense strand SNF1 antisense strand 3' 5 2. What are...