Question

The table shows where different restriction endonucleases (restriction enzymes) cleave DNA. The abbreviation R represents the purines (adenine and guanine). The pyrimidines (cytosine, thymine, and uracil) are abbreviated as Y. The abbreviation W represents adenine or thymine. Enzyme EcoRI EcoRV Target sequence 5' GAATTC 3 3' CTTAAG 5 5' GATATC 3 3' CTATAG 5 5' GGCC 3' 3' CCGG 5 5' AAGCTT 3 3' TTCGAA 5 5' RGGWCCY 3 3' YCCWGGR 5 Cleavage 5G AATTC 3' 3' CTTAA G 5' 5' GAT ATC 3 3' CTA TAG 5 5' GG CC 3 3' CC GG 5 5'A AGCTT 3 3' TTCGA A 5 5'RG GWCCY 3' 3' YCCWG GR 5' HaeIII HindIII PpuMI Which restriction endonuclease(s) would cleave a DNA molecule at the given sequences? a) 5' ATCGAACTAGGCC 3' b) 5' TCCAAGCTTGAATTC 3 EcoRI HindIII EcoRV HaeIII HaeIII EcoRV HindIII EcoRI c) Plasmid DNA and a gene of interest are cut with the enzyme PpuMI. Write a possible sequence of bases for the sticky end of the gene in the 5' to 3' direction. possible sequence of bases: 5' GWC 3'

Answer 1

Answer

a) Option D is correct i.e " HaeIII"

Explanation

The nucleotide sequence i.e " 5' ATCGAACTAGGCC 3' and the recognition site of Hae III enzyme is 5'GGCC3' and this site is present in the mentioned sequence " 5' ATCGAACTAGGCC 3'"

b) Option C and D both are correct i.e " HindIII & ECORI"

Explanation

The nucleotide sequence i.e " 5' TCCAAGCTTGAATTC 3' " and the recongnition site of Hind III enzyme is AAGCTT and the recognition site of ECORI enzyme is GAATTC and these sites are present in the mentioned sequence " " 5' TCCAAGCTTGAATTC 3' "

Answer (c)

The possible sequence of bases are " 5' GWC 3' where W can adenine or thymine so possible sequences are

5' GAC 3' or 5' GTC 3'.