How do you increase the resolution/ separation of a cation exchange chromatography? Explain why.
For an cation exchange chromatographic separation, enhancement of resolution of separation can be easily achieved by increasing the pH slowly for the elution part. This was the difference between the pI values would be increased and thus resolution would also increase accordingly.
How do you increase the resolution/ separation of a cation exchange chromatography? Explain why.
chromatography mechanisms -In ion-exchange mechanism of chromatography, let assume we use cation exchangers, cation will attract to stationary phase and remain binded untill we change th PH to get that cation my question is what I do with PH, raised it decrease it to get the cation? and why? -the same for the covalently binding of protein and antigen in affinity chromatography mechanism, how can I get the protein from antigen? is it by using PH (increa decrease)? of course,...
Could you please explain how ion exchange chromatography works and how the anion exhange chromatography relates? Thanks!
How can you improve the resolution between two closely spaced peaks in gas chromatography. Describe the process you would undertake to improve separation.
Besides cation-exchange chromatography what other seperation techniques you could use to analyse a mixture of amino acids?
Explain the various factors responsible for separation of components of a mixture in chromatography. Explain about the classification of chromatography with few examples. Draw a block diagram of gas chromatograph and explain the function of each component Write a note on the micro syringe use to inject the liquid sample into gas chromatograph. Write a note on principle of working of Gas chromatograph. (How to prepare GC for analysis) Write a note on analysis of chromatogram (Types the method of...
Why is characterization of complexes trans-CrCl2(OH2)4+, CrCl(OH2)52+, and Cr(OH2)63+ following cation exchange chromatography done by visible spectroscopy rather than IR spectroscopy?
(3 points) A Student uses cation exchange chromatography to separate Protein 1 pi9.5) and Protein 2 pl 45). The column is equilibrated using a 20m phosphate buffer, pH 6.0 and then the sample containing Protein 1 and Protein 2 is applied to the column. After the sample is applied to the column, the column is first washed with a low salt buffer. Subsequently, the column is washed with a high salt buffer. The elution profile is shown below. Clearly indicate...
Please answer What are some factors that could affect the selectivity and the resolution in Gas Chromatography (GC) & Liquid Chromatography (LC), and How do we affect the separation in (GC)&(LC) ?
In cation exchange chromatography, the beads are covalently linked to a ________chemical and a ______________ ion is used as the counter-ion. a. positive, negative b. negative, positive c. negative, negative d. positive, positive Which of the following is NOT an application of a DNA microarray? a. SNP detection b. comparative genomic hybridization c. gene expression profiling d. alternative splicing detection e. imprinting Individuals susceptible to tumorigenesis have an abundance of receptors specific for: a. serotonin b. testosterone c. acetylcholine d....
In gas chromatography how does column length, temperature, and coating thickness affect the resolution?