Besides cation-exchange chromatography what other seperation techniques you could use to analyse a mixture of amino...

Question

Question

Besides cation-exchange chromatography what other seperation techniques you could use to analyse a mixture of amino acids?

Answer 1

Answer #1

these are the above 4 mentioned techniques for the separation of amino acid mixtures other than ion exchange chromatography

Answer 2

Similar Homework Help Questions

chromatography mechanisms -In ion-exchange mechanism of chromatography, let assume we use cation exchangers, cation will attract...

chromatography mechanisms -In ion-exchange mechanism of chromatography, let assume we use cation exchangers, cation will attract to stationary phase and remain binded untill we change th PH to get that cation my question is what I do with PH, raised it decrease it to get the cation? and why? -the same for the covalently binding of protein and antigen in affinity chromatography mechanism, how can I get the protein from antigen? is it by using PH (increa decrease)? of course,...
14) Which of the following amino acids will by cation-exchange chromatography? following amino acids will be...

14) Which of the following amino acids will by cation-exchange chromatography? following amino acids will be retained longest in the column when sepa Η serne HO-CH-C-COO v. Lysine H3N-CH2CH2CH2CH2CH-co NES NH V. Phenylalanine CH-C–COOH II. Alanine CH3-C-Coo II. Cysteine HS-CH-c-coo® NE 15) What is the product obtained from mild oxidation of the thiol shown below? mild 2R-SH oxidation A) R-S- R B) R-OH C) R-S-S-R DR-OO- RE ) R-S-OH 16) Which bond in the following compound is a peptide bond?...
A student used chromatography to separate a mixture of amino acids. Since amino acids are invisible...

A student used chromatography to separate a mixture of amino acids. Since amino acids are invisible on the paper, what can be done to make them visible?

Ion Exchange Chromatography Experiment 1. What is the basis for the seperation of different compounds by...

Ion Exchange Chromatography Experiment 1. What is the basis for the seperation of different compounds by ion exchange? 2. How can molecules with the same charge at varying amounts be seperated by chromatography? 3. Why are celluloses often used as supports to seperate large biologically active proteins? 4. Why is it important to prepare a standard curve for each spectrophotometer? 5. What would happen if 0.5M K+ acetate were used to elute the sample? Why? (0.01M KOAc was actually used)
How do you increase the resolution/ separation of a cation exchange chromatography? Explain why.

How do you increase the resolution/ separation of a cation exchange chromatography? Explain why.
How would you use ion exchange chromatography to seperate a mixture of alanine, glutamic acid, and...

How would you use ion exchange chromatography to seperate a mixture of alanine, glutamic acid, and lysine if you wished to maintain the pH at 7.5 and use only a single ion exchange column ( either an anionic or cationic exchanger). Describe the expected order of elution under the conditions which you propose.

please explain thoroughly and show all work If you applied an amino acid mixture containing Histidine...

please explain thoroughly and show all work If you applied an amino acid mixture containing Histidine and Lysine onto ion exchange chromatography, at what pH should the chromatography be performed? Explain your reasoning. Lysine: pKa1-2.2, pKa2-9.1 and pKaR- 10.5 Histidine: pKa1=1.8, pKa2-9.3 and pKaR-6.0 If you applied an amino acid mixture containing Histidine and Lysine onto ion exchange chromatography, at what pH should the chromatography be performed? Explain your reasoning. Lysine: pKa1-2.2, pKa2-9.1 and pKaR- 10.5 Histidine: pKa1=1.8, pKa2-9.3 and...
Could you please explain how ion exchange chromatography works and how the anion exhange chromatography relates?...

Could you please explain how ion exchange chromatography works and how the anion exhange chromatography relates? Thanks!
D. CHROMATOGRAPHY Chromatography is a general technique that is used to separate various materials. In this case,...

D. CHROMATOGRAPHY Chromatography is a general technique that is used to separate various materials. In this case, students can use the unique chemistry of the amino acid to determine its migration in a specific solvent. The solutions of amino acids can be applied to the place and allowed to dry. The plate is placed in the solvent, and capillary action will carry the amino acids upward along the plate. The side chains, or R groups, can be characterized by their...

What are the advantages and disadvantages gas chromatography (hint: compare to other chromatographic techniques we have...

What are the advantages and disadvantages gas chromatography (hint: compare to other chromatographic techniques we have investigated)?