Question

5'-GCA CAG GCT ACC CCC CCC CCC CTA GCC TGT GC-3' Determine the melting point assuming only the hair pin is created ( no complimentary strand ). The spaces are just for easier reading.

Answer 1

the chemichal formula is ;

Td = 2 degree [ A+T] + 4 degree { G + c}

= 2 [ 4+ 4] + 4{ 6+ !8}

=16+ 96

= 112 degree is the melting point temperature

if there is hair pin loops then the melting temperature is 74.6