Homework Answers
Answers
2) Lysis of cells
Becuase the steps involved in the DNA isolation is
a) Lysis of cells
b) Precipitation
c) Purification
3) Upper aqueous
Because in the DNA extraction method the DNA is found in the upper aqueous .
4)Isopropanol
DNA after seperation from other cellular constituent is precipitated with isopropanol.
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2- Which of the following is the first step in DNA isolation from cells in a...
2- Which of the two DNAs A and B are pure. Explain your choice. 17.90 16.00...
2- Which of the two DNAs A and B are pure. Explain your choice. 17.90 16.00 9.00 ON Phenol 14.00 DNA 12.00 10.00 10 mm Absorbance 10 mm Absorbance 0.00 220 230 240 250 260 270 280 290 300 310 320 330 340 350 Wavelength im! 220 230 240 250 260 270 280 280 560 510 320 330 340 360 Wavelength ihm) Choose the correct answer. Only one response 1- Which of the pipettes above is set to dispense 32.7...
What solution is used to isolate the DNA from the bacterial cells 7. Alkaline lysis buffer...
What solution is used to isolate the DNA from the bacterial cells 7. Alkaline lysis buffer b Acidic lysis buffer RNase C d None of the above 8 What solution is used to isolate the DNA from the spin column? DNA buffer a. b. Elution buffer P1 c. d. P2 9 EcoRI restriction site is 5' GAATTC 3' and 3' CTTAAG 5 a 5' GAATTC 3' and 3' CTTAAG 5 b 5' GAATTC 3' and 3' CTTAAG 5 C. d....
Attached below are the pages from this lab report to help give context to the questions....
Attached below are the pages from this lab report to help give
context to the questions. Please be as detailed as possible when
answering the questions and include any relevant information about
intermolecular forces at play. Thank you for your help!
Here are the questions which I need answered. Thank you in
advance for your help! :) Please be as detailed as possible and
discuss any intermolecular forces in detail if they are relevant to
the question. Thanks!
PARTA. SEPARATION...
En (2 points) You isolated your mitochondrial DNA in Part I. In step 6, you discard...
En (2 points) You isolated your mitochondrial DNA in Part I. In step 6, you discard the supernatant, but keep the pellet. In step 15, you discard the pellet, but keep the supernatant. Explain why the pattern is different between the two steps and the consequence of mixing up these two steps. Procedure Part 1: mt DNA Isolation from your cheek cells. Lysis solution is used to breakdown the cells in this step, you will isolate MEONA from cheek cells....
Construct a flow chart describing the seperation of the mixture and the isolation of each compound...
Construct a flow chart describing the seperation of the
mixture and the isolation of each compound in this experiment. (Lab
steps/procedures includes for reference)
4. Construct a flow chart describing the separation of the mixture and the isolation of each compound in this experiment. A commonly used method of separating a mixture of organic compounds is known as liquid-liquid extraction. Most reactions of organic compounds require extraction at some stage of product purification. In this experiment you will use extraction...
42. Which statement is NOT true about DNA? a) DNA acts like an enzyme by binding...
42. Which statement is NOT true about DNA? a) DNA acts like an enzyme by binding amino acids together using dehydration synthesis. b) Before cells divide both strands must be copied and one is provided for each new cell cm RNA is copied from a piece of DNA termed a gene. d) DNA is not always copied perfectly. Mistakes, mutations, occur and result in changed proteins, e) DNA functions as code for the synthesis of proteins. 43. A section of...
For part 1 of this lab) I collected a soil sample from my campus Part 2)...
For part 1 of this lab) I collected a soil sample from my campus
Part 2) Tested bacteria initial viability Part 3) DNA extraction
Part 4) DNA quantification by Nanodrop Part 5) Sample sequencing
Part 6) PCR amplification Part 7) Gel electrophoresis Part 8) DNA
sequence data analysis (sent sample to another lab)
Directions for Part 3 DNA extraction are in the attached
image
QUESTIONS REGARDING PART 3 (DNA extraction)
1) What type of conclusions can be made from initially...
i need help in biochem Which of the following statements is true? Exonuclease III cleaves DNA...
i need help in biochem Which of the following statements is true? Exonuclease III cleaves DNA in a sequence specific manner Bacteriophage lambda exonuclease adds homopolymer tails to the 3’ –OH ends of a linear duplex Alkaline phosphatase removes terminal phosphates Kinase removes nucleotides from 5‘ends (5 points) Arrange the following steps involved in Gene cloning in the correct order a) Expression of recombinant gene in host b) Insertion of isolated gene to the vector c) Extraction of recombinant gene...
1. Describe the functions of the following reagents in extraction of DNA from corn meal: proteina...
1. Describe the functions of the following reagents in extraction of DNA from corn meal: proteinase K; guanidine HCI; SDS 2. Why is the ratio of the OD at 260 and 280 nm used to estimate DNA purity? 3. In one paragraph, summarize basic principles of PCR technique in your own words. List all the reagents you will need to perform a PCR experiment. Does this method tell you what genetic modifications were made? If yes, describe how you can...
DNA, Genes and Protein Synthesis Activity 13: Protein Synthesis is the process by which cells produce (synthesize) proteins. An overview of the process is shown in model 2 (below). Gone 2...
DNA, Genes and Protein Synthesis Activity 13: Protein Synthesis is the process by which cells produce (synthesize) proteins. An overview of the process is shown in model 2 (below). Gone 2 Gene 1 Gene 3 DNA strand3 TRANSLATION Protein Trp Gly Model 2 ACTIVITY and QUESTIONS 1. Based on the information you can gather from model 1 complete the following sentences: a. The nucleotide Adenine (A) always pairs with the nucleotide b. The nucleotide Guanine (G) always pairs with the...
2- Which of the two DNAs A and B are pure. Explain your choice. 17.90 16.00 9.00 ON Phenol 14.00 DNA 12.00 10.00 10 mm Absorbance 10 mm Absorbance 0.00 220 230 240 250 260 270 280 290 300 310 320 330 340 350 Wavelength im! 220 230 240 250 260 270 280 280 560 510 320 330 340 360 Wavelength ihm) Choose the correct answer. Only one response 1- Which of the pipettes above is set to dispense 32.7...
What solution is used to isolate the DNA from the bacterial cells 7. Alkaline lysis buffer b Acidic lysis buffer RNase C d None of the above 8 What solution is used to isolate the DNA from the spin column? DNA buffer a. b. Elution buffer P1 c. d. P2 9 EcoRI restriction site is 5' GAATTC 3' and 3' CTTAAG 5 a 5' GAATTC 3' and 3' CTTAAG 5 b 5' GAATTC 3' and 3' CTTAAG 5 C. d....
Attached below are the pages from this lab report to help give
context to the questions. Please be as detailed as possible when
answering the questions and include any relevant information about
intermolecular forces at play. Thank you for your help!
Here are the questions which I need answered. Thank you in
advance for your help! :) Please be as detailed as possible and
discuss any intermolecular forces in detail if they are relevant to
the question. Thanks!
PARTA. SEPARATION...
En (2 points) You isolated your mitochondrial DNA in Part I. In step 6, you discard the supernatant, but keep the pellet. In step 15, you discard the pellet, but keep the supernatant. Explain why the pattern is different between the two steps and the consequence of mixing up these two steps. Procedure Part 1: mt DNA Isolation from your cheek cells. Lysis solution is used to breakdown the cells in this step, you will isolate MEONA from cheek cells....
Construct a flow chart describing the seperation of the
mixture and the isolation of each compound in this experiment. (Lab
steps/procedures includes for reference)
4. Construct a flow chart describing the separation of the mixture and the isolation of each compound in this experiment. A commonly used method of separating a mixture of organic compounds is known as liquid-liquid extraction. Most reactions of organic compounds require extraction at some stage of product purification. In this experiment you will use extraction...
42. Which statement is NOT true about DNA? a) DNA acts like an enzyme by binding amino acids together using dehydration synthesis. b) Before cells divide both strands must be copied and one is provided for each new cell cm RNA is copied from a piece of DNA termed a gene. d) DNA is not always copied perfectly. Mistakes, mutations, occur and result in changed proteins, e) DNA functions as code for the synthesis of proteins. 43. A section of...
For part 1 of this lab) I collected a soil sample from my campus
Part 2) Tested bacteria initial viability Part 3) DNA extraction
Part 4) DNA quantification by Nanodrop Part 5) Sample sequencing
Part 6) PCR amplification Part 7) Gel electrophoresis Part 8) DNA
sequence data analysis (sent sample to another lab)
Directions for Part 3 DNA extraction are in the attached
image
QUESTIONS REGARDING PART 3 (DNA extraction)
1) What type of conclusions can be made from initially...
1. Describe the functions of the following reagents in extraction of DNA from corn meal: proteinase K; guanidine HCI; SDS 2. Why is the ratio of the OD at 260 and 280 nm used to estimate DNA purity? 3. In one paragraph, summarize basic principles of PCR technique in your own words. List all the reagents you will need to perform a PCR experiment. Does this method tell you what genetic modifications were made? If yes, describe how you can...
DNA, Genes and Protein Synthesis Activity 13: Protein Synthesis is the process by which cells produce (synthesize) proteins. An overview of the process is shown in model 2 (below). Gone 2 Gene 1 Gene 3 DNA strand3 TRANSLATION Protein Trp Gly Model 2 ACTIVITY and QUESTIONS 1. Based on the information you can gather from model 1 complete the following sentences: a. The nucleotide Adenine (A) always pairs with the nucleotide b. The nucleotide Guanine (G) always pairs with the...
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