The final reaction of glycolysis is catalyzed by pyruvate kinase and has a ΔG’° of -31.4kJ/mol....
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The final reaction of glycolysis is catalyzed by pyruvate kinase and has a ΔG’° of -31.4kJ/mol. In vivo this enzyme has a ΔG of -16.7kJ/mol. In gluconeogenic tissues like the liver, the cytosolic concentrations of ATP, ADP, Pi and PEP are 2.25mM, 0.25mM, 1.65mM and 8.8μM respectively. Assuming everything else remains constant, what fold change in pyruvate concentration would be required to drive pyruvate kinase in the reverse direction? (That is, solve the equation when ΔG is 0). |
Homework Answers
ADP+ Pi = ATP (G is positive
means it is the reverse process)
G0 =
G0p
-
G0r (summation of
products & reactants)
=2.25 - ( 0.25 + 1.65) = 2.25 - 1.90 = 0.35kJ/mol
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The final reaction of glycolysis is catalyzed by pyruvate kinase
and has a ΔG’° of -31.4kJ/mol....
5.The following reaction has a ΔG°'--61.9 kJ/mol at 25°C, Phosphoenolpyruvate (PEP) +H2O Pyruvate + Inorganic Phosphate...
5.The following reaction has a ΔG°'--61.9 kJ/mol at 25°C, Phosphoenolpyruvate (PEP) +H2O Pyruvate + Inorganic Phosphate (Pi) (a) Is the reaction favorable under standard biochemical conditions? Explain your answer. (b) What is the equilibrium constant (K) of this reaction at 25°C? In cells the direct hydrolysis of PEP rarely occurs. Rather, the hydrolysis of PEP and the reverse hydrolysis of ATP are coupled (c) w rite out the coupled reaction and determine its ΔG at 25°C The hydrolysis of ATP...
Consider the reaction catalyzed by the glycolytic enzyme pyruvate kinase. Is this reaction simply reversed in...
Consider the reaction catalyzed by the glycolytic enzyme pyruvate kinase. Is this reaction simply reversed in the gluconeogenic pathway? Carefully describe the chemical reaction(s), enzyme(s) involved, and bioenergetics of the "reverse" of the pyruvate kinase reaction; in other words, how does pyruvate get converted back into phosphoenolpyruvate when gluconeogenesis is upregulated?
The reaction catalysed by pyruvate kinase is: K'eg 3.63 x 105 Phosphoenolpyruvate + ADP -> pyruvate ATP a) Calculate the AG°" for this reaction. Show your working. 3 marks b) The hydrolysi...
The reaction catalysed by pyruvate kinase is: K'eg 3.63 x 105 Phosphoenolpyruvate + ADP -> pyruvate ATP a) Calculate the AG°" for this reaction. Show your working. 3 marks b) The hydrolysis of ATP has following equation: ATP+H20ADP P AG-30.5 kJ/mol Calculate the AG" for the following reaction: Phosphoenolpyruvate -> pyruvate Pi Show your working. 2 marks c) At 37 °C, the steady-state concentrations of phosphoenolpyruvate, ATP, and ADP have been measured to be 23 μΜ, 1.85 mM and 140...
Pyruvate kinase catalyzes the last reaction in the glycolytic pathway: phosphoenolpyruvate (PEP) + ADP --> pyruvate...
Pyruvate kinase catalyzes the last reaction in the glycolytic
pathway:
phosphoenolpyruvate (PEP) + ADP --> pyruvate + ATP
There are four different mammalian forms of pyruvate kinase. All
catalyze the same reaction, but they differ in their response to
the glycolytic metabolite fructose-1,6-bisphosphate (F16BP).
The activity of two forms of pyruvate kinase was measured at
various concentrations of PEP, as shown below. Activity was
measured in the presence (blue circles) and absence (red circles)
of F16BP.
Pyruvate Kinase Form 1...
Pyruvate kinase catalyzes the last reaction in the glycolytic pathway: phosphoenolpyruvate (PEP) + ADP --> pyruvate...
Pyruvate kinase catalyzes the last reaction in the glycolytic
pathway:
phosphoenolpyruvate (PEP) + ADP --> pyruvate + ATP
There are four different mammalian forms of pyruvate kinase. All
catalyze the same reaction, but they differ in their response to
the glycolytic metabolite fructose-1,6-bisphosphate (F16BP).
The activity of two forms of pyruvate kinase was measured at
various concentrations of PEP, as shown below. Activity was
measured in the presence (blue circles) and absence (red circles)
of F16BP.
Pyruvate Kinase Form 1...
like me to grade. I vow did the take homeow 1. In glycolysis, the enzyme pyruvate...
like me to grade. I vow did the take homeow 1. In glycolysis, the enzyme pyruvate the enzyme pyruvate kinase catalyzes this reaction phosphoenolpyruvate + ADP - pyruvate + ATP caction using the information provided a. What is the equilibrium constant for below? (R-8.315 J/mol K; T = 298 K) Reaction 1: ATP - ADP +P Reaction 2: phosphoenolpyruvate vale pyruvate + PAG AG"=-30.5 kJ/mol pyruvate + P AGⓇ = -61.9 kJ/mol ntrations are provided below, what is the actual...
1. 2. 3. 4. Select all that apply. Identify the reaction(s) of glycolysis inhibited by ATP □ glyceraldehyde-3-phosphate → 1,3-bisphosphoglycerate phosphoenolpyruvate +pyruvate 3-phosphoglycerate...
1.
2.
3.
4.
Select all that apply. Identify the reaction(s) of glycolysis inhibited by ATP □ glyceraldehyde-3-phosphate → 1,3-bisphosphoglycerate phosphoenolpyruvate +pyruvate 3-phosphoglycerate ~2-phosphoglycerate 1) fructose-6-phosphate→ fructose-1,6-bisphosphate . glucose glucose-6-phosphate Select all that apply. Alcohol fermentation: consumes ATP as it produces ethanol. O produces carbon dioxide begins as pyruvate is decarboxylated to acetaldehyde in a reaction catalyzed by alcohol dehydrogenase m regenerates NAD requires the enzyme alcohol dehydrogenase, In the figure below, what is the reaction occurring at the location...
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Question 1 - Linked Reactions (10 marks) The reaction catalysed by pyruvate kinase is: Phosphoenolpyruvate+ ADPpyruvateATP Keg 3.63 x 105 a) Calculate the AG" for this reaction. Show your work...
Question 1 - Linked Reactions (10 marks) The reaction catalysed by pyruvate kinase is: Phosphoenolpyruvate+ ADPpyruvateATP Keg 3.63 x 105 a) Calculate the AG" for this reaction. Show your working. 3 marks b) The hydrolysis of ATP has following equation: AG 30.5 kJ/mol Calculate the AG" for the following reaction: Phosphoenolpyruvate pyruvateP Show your working. 2 marks c) At 37 °C, the steady-state concentrations of phosphoenolpyruvate, ATP, and ADP have been measured to be 23 HM, 1.85 mM and 140...
5.The following reaction has a ΔG°'--61.9 kJ/mol at 25°C, Phosphoenolpyruvate (PEP) +H2O Pyruvate + Inorganic Phosphate (Pi) (a) Is the reaction favorable under standard biochemical conditions? Explain your answer. (b) What is the equilibrium constant (K) of this reaction at 25°C? In cells the direct hydrolysis of PEP rarely occurs. Rather, the hydrolysis of PEP and the reverse hydrolysis of ATP are coupled (c) w rite out the coupled reaction and determine its ΔG at 25°C The hydrolysis of ATP...
The reaction catalysed by pyruvate kinase is: K'eg 3.63 x 105 Phosphoenolpyruvate + ADP -> pyruvate ATP a) Calculate the AG°" for this reaction. Show your working. 3 marks b) The hydrolysis of ATP has following equation: ATP+H20ADP P AG-30.5 kJ/mol Calculate the AG" for the following reaction: Phosphoenolpyruvate -> pyruvate Pi Show your working. 2 marks c) At 37 °C, the steady-state concentrations of phosphoenolpyruvate, ATP, and ADP have been measured to be 23 μΜ, 1.85 mM and 140...
Pyruvate kinase catalyzes the last reaction in the glycolytic
pathway:
phosphoenolpyruvate (PEP) + ADP --> pyruvate + ATP
There are four different mammalian forms of pyruvate kinase. All
catalyze the same reaction, but they differ in their response to
the glycolytic metabolite fructose-1,6-bisphosphate (F16BP).
The activity of two forms of pyruvate kinase was measured at
various concentrations of PEP, as shown below. Activity was
measured in the presence (blue circles) and absence (red circles)
of F16BP.
Pyruvate Kinase Form 1...
Pyruvate kinase catalyzes the last reaction in the glycolytic
pathway:
phosphoenolpyruvate (PEP) + ADP --> pyruvate + ATP
There are four different mammalian forms of pyruvate kinase. All
catalyze the same reaction, but they differ in their response to
the glycolytic metabolite fructose-1,6-bisphosphate (F16BP).
The activity of two forms of pyruvate kinase was measured at
various concentrations of PEP, as shown below. Activity was
measured in the presence (blue circles) and absence (red circles)
of F16BP.
Pyruvate Kinase Form 1...
like me to grade. I vow did the take homeow 1. In glycolysis, the enzyme pyruvate the enzyme pyruvate kinase catalyzes this reaction phosphoenolpyruvate + ADP - pyruvate + ATP caction using the information provided a. What is the equilibrium constant for below? (R-8.315 J/mol K; T = 298 K) Reaction 1: ATP - ADP +P Reaction 2: phosphoenolpyruvate vale pyruvate + PAG AG"=-30.5 kJ/mol pyruvate + P AGⓇ = -61.9 kJ/mol ntrations are provided below, what is the actual...
1.
2.
3.
4.
Select all that apply. Identify the reaction(s) of glycolysis inhibited by ATP □ glyceraldehyde-3-phosphate → 1,3-bisphosphoglycerate phosphoenolpyruvate +pyruvate 3-phosphoglycerate ~2-phosphoglycerate 1) fructose-6-phosphate→ fructose-1,6-bisphosphate . glucose glucose-6-phosphate Select all that apply. Alcohol fermentation: consumes ATP as it produces ethanol. O produces carbon dioxide begins as pyruvate is decarboxylated to acetaldehyde in a reaction catalyzed by alcohol dehydrogenase m regenerates NAD requires the enzyme alcohol dehydrogenase, In the figure below, what is the reaction occurring at the location...
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