Question

A sample of pond sediment was diluted by placing a 0.1 g into 9.9 ml of...

A sample of pond sediment was diluted by placing a 0.1 g into 9.9 ml of diluent, and 0.1 ml of this
dilution was spread to two Nutrient Agar (NA) plates. After 3 d incubation, 186 colonies were counted
on one plate and 198 on the other. What is the CFU/g of the sediment? (Assume that the density of the
sediment is about that of water)
0 0
Add a comment Improve this question Transcribed image text
Answer #1

The density of the sediment is about that of water = 1gm/ml

Therefore, when 0.1 gm of sediment was diluted into 9.9ml of diluent,

Dilution factor=0.1 gm x 1 gm/ml / 10 ml (Total volume of solution) = 0.01

CFU/gm = No of colonies / Dilution factor = 198 / 0.01 = 19800

CFU/gm = No of colonies / Dilution factor = 186 / 0.01 = 18600

If we take the average = (19800+18600) / 2 = 19200 CFU/gm

Add a comment
Know the answer?
Add Answer to:
A sample of pond sediment was diluted by placing a 0.1 g into 9.9 ml of...
Your Answer:

Post as a guest

Your Name:

What's your source?

Earn Coins

Coins can be redeemed for fabulous gifts.

Not the answer you're looking for? Ask your own homework help question. Our experts will answer your question WITHIN MINUTES for Free.
Similar Homework Help Questions
  • What is the equation for calculating the cfu/ g soil? We used 1g soil and diluted...

    What is the equation for calculating the cfu/ g soil? We used 1g soil and diluted it in 9mL of water --> is considered to be our 10-1 dilution. --> dilution factor is 10 We plated 0.1 mL of this 10-1 solution onto an agar plate. Can I use following equation to calculate the cfu/ g soil (density is neglected): cfu/g soil=(no.of colonies x dilution factor)/(volume of culture plate) cfu/g soil =( 30 cfu x 10) / 0.1 mL =...

  • Hello, just wanted to check my answers. Answer as many as you like/can. Thankyou. 1) Four...

    Hello, just wanted to check my answers. Answer as many as you like/can. Thankyou. 1) Four grams of soil are added to 36 ml sterile water and mixed well. 0.1 ml of this mix was added to 9.9 ml sterile water. This was diluted by 4 successive 1/10 dilutions. 1.0 ml from the last dilution was used to prepare a pour plate. After incubation 289 colonies were counted. Calculate the # CFU/g soil. 2) A 0.1 ml aliquot of a...

  • You spread 0.1 mL volume of a 10^(-5) dilution onto a nutrient agar plate. After 24...

    You spread 0.1 mL volume of a 10^(-5) dilution onto a nutrient agar plate. After 24 hours of incubation at 37°C, there were 223 colonies of bacteria on the plate. A.) What is the original concentration (OCD) of bacteria in the stock sample this dilution came from? (2 points) B.) Using the OCD value from part A, determine the number of colonies that would be expected to grow on a plate that is inoculated with 0.1 mL volume of 10^(-7)...

  • A. You have been given a tube of E. coli. You are asked to make 1...

    A. You have been given a tube of E. coli. You are asked to make 1 mL total volume of 10-1 dilution of the bacterial culture. Explain how you would do this. Show all necessary calculations. ____ ml cells   +    _____ ml water    = 1 ml    (total volume) B. Next, you were asked to make a 10-2 dilution of the bacterial sample. Explain how you would perform this. Show all necessary calculations. You have bacteria at a concentration of 2...

  • 2. A series of 1 ml samples from tube #4 of the dilution s eries below are spread on agar plates,...

    2. A series of 1 ml samples from tube #4 of the dilution s eries below are spread on agar plates, resulting in the growth of an average of 68 colonies per plate. What is the concentration of colony-forming units (cfu/mi) present in the original culture? 0.1 ml 0.1 ml 1 ml 1 ml 100 ml 9.9 ml 9.9 ml 9 ml 9 ml 2 4 3. The culture flask at left contains a total of 3.2 x 10s cfu....

  • A bacterial sample has a concentration of 2 x106 CFUlml. Show a scheme of dilutions to...

    A bacterial sample has a concentration of 2 x106 CFUlml. Show a scheme of dilutions to obtain 30-300 colonies on a plate. Your scheme should contain the volume of diluent (sterile water), volume of sample transferred each time, the concentration of bacteria (CFU/mL) in each dilution. You can assume you are plating 1 mL of the dilution on plates of nutrient agar. See Figure 1 in protocol 10. Dilution factor in single transfer (change x and y to numbers 10...

  • You spread 0.1 mL volume of a 10^(-6) dilution onto a nutrient agar plate. After 24...

    You spread 0.1 mL volume of a 10^(-6) dilution onto a nutrient agar plate. After 24 hours of incubation at 37°C, there were 280 colonies of bacteria on the plate. A.) What is the original concentration (OCD) of bacteria in the stock sample this dilution came from? (2 points) B.) Using the OCD value from part A, determine the number of colonies that would be expected to grow on a plate that is inoculated with 0.1 mL volume of 10^(-8)...

  • please help with whatever possible. thank you so much in advance. Name One use of serial...

    please help with whatever possible. thank you so much in advance. Name One use of serial dilutions is to calculate the concentration of microorganisms. Since it would usually be challenging or even impossible to actually count the number of microorganisms in a sample, the sample is diluted and plated to get a reasonable number of colonies to count (usually between 25 to 250 colonies is the goal). Since each colony on an agar plate theoretically grew from a single microorganism,...

  • 9. A. You have diluted a sample by 1000 fold and plated 1 mL on an...

    9. A. You have diluted a sample by 1000 fold and plated 1 mL on an agar plate. You observe 35 colonies. What was the concentration of the original sample in CFU/mL?

  •             A urine sample is diluted 1:100. 200 µl of the 1:100 dilution is spread onto an...

                A urine sample is diluted 1:100. 200 µl of the 1:100 dilution is spread onto an agar plate. After incubation, 250 colonies are observed. What is the concentration of bacteria in the original urine sample? Show your work and include proper units for full credit.

ADVERTISEMENT
Free Homework Help App
Download From Google Play
Scan Your Homework
to Get Instant Free Answers
Need Online Homework Help?
Ask a Question
Get Answers For Free
Most questions answered within 3 hours.
ADVERTISEMENT
ADVERTISEMENT
ADVERTISEMENT