If we can use spectrophotometer reading to calculate the CFU/ml for the growth curve, why didn't...
If we can use spectrophotometer reading to calculate the CFU/ml for the growth curve, why didn't we use the spectrophotometer for the DRT curve?
Homework Answers
The DRT curve is the decimal reduction time in which 90% of apopulation is killed at a specific temperature. The spectrophotometer will not be able to show the 10% properly. The spectrophotometer method depends mainly on the turbidity of the solution for calculating cfu/ml. Since 10% of microbes will not show much of turbidity, the reading may come as negligible or no organisms. In calculating cfu/ml, the turbidity of the solution gradually increases. This helps in calculation of the growth of organisms.
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If we can use spectrophotometer reading to calculate the CFU/ml
for the growth curve, why didn't...
What is the equation for calculating the cfu/ g soil? We used 1g soil and diluted...
What is the equation for calculating the cfu/ g soil? We used 1g soil and diluted it in 9mL of water --> is considered to be our 10-1 dilution. --> dilution factor is 10 We plated 0.1 mL of this 10-1 solution onto an agar plate. Can I use following equation to calculate the cfu/ g soil (density is neglected): cfu/g soil=(no.of colonies x dilution factor)/(volume of culture plate) cfu/g soil =( 30 cfu x 10) / 0.1 mL =...
this is a bacterial growth curve experiment . please explain rhe results Procedure: You will follow...
this is a bacterial growth curve experiment . please explain
rhe results
Procedure: You will follow the growth of E. coli over the course of the period (3 hrs) by making direct counts of the bacterial suspension by measuring the turbidity of a sample at a given time with a spectrophotometer. The data obtained from the direct counts will be used to plot a partial growth curve. Summary: Turbidity Counts with the Spectrophotometer to measure absorbance at 600. Direct Counts...
3. You are measuring the growth of bacterium using cfu mL·You collect samples at various times an...
3. You are measuring the growth of bacterium using cfu mL·You collect samples at various times and dilute them in 10-fold series (as shown in the 2 row of the table) and plate 0.1 ml of each of these dilutions on five standard lab agar plates. The average number of colonies per plate are below Time of Colonies on plate at each dilution ars 45 290 TNTC TNTC 195 20 TNTC TNTC 200 TNTC too numerous to count ADB MO...
growth rates provide his whole information because they can tell us something about optimum growth conditions....
growth rates provide his whole information because they can
tell us something about optimum growth conditions. For example if
you were a plot a series of growth curves at various temperatures
and calculate the growth rates, you could generate a graph similar
to the one at the right.
what is the original growth curve in part 1 obtained at the
optimal temperature for this organism? If yes, explain how you
knew. if not, can you tell at what temperature the...
Table 6.3. Data Used to construct Figure 6.10 and 6.11. Hour Absorbance Cfu/ml 0.16 x 10 0.16 x 1...
bacterial growth curve help
whats wrong with each graph using the chart provided.
Table 6.3. Data Used to construct Figure 6.10 and 6.11. Hour Absorbance Cfu/ml 0.16 x 10 0.16 x 10 0.16 x 10 0.16 x 10 0.78 x 10 1.98 x 10 2.53 x 10% 3.13 x 10% 4.38 x 10 5.97× 106 9.41 x 10 11.9 x 10 14.7 x 10 21.0× 106 22.0× 106 22.2 x 10% 0.01 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0...
3. You are measuring the growth of bacterium using cfu mL·You collect samples at various times...
3. You are measuring the growth of bacterium using cfu mL·You collect samples at various times and dilute them in 10-fold series (as shown in the 2 row of the table) and plate 0.1 ml of each of these dilutions on five standard lab agar plates. The average number of colonies per plate are below Time of Colonies on plate at each dilution ars 45 290 TNTC TNTC 195 20 TNTC TNTC 200 TNTC too numerous to count ADB MO...
explain what are critical reading skills and why do we use them?
explain what are critical reading skills and why do we use them?
What are 'diluted standards' and why do we use them? A) Cuvettes with known concentrations of...
What are 'diluted standards' and why do we use them? A) Cuvettes with known concentrations of the substance, analysed to provide a calibration curve. B) Cuvettes filled with concentrations that will fall below the detection limit of the spectrophotometer, to ensure there is no noise in the data C) Cuvettes filled with solutions to rinse each cuvette before analysis takes place D) Cuvettes with various standard substances analysed so that the unknown molecule(s) can be identified
What would be some reasons as to why a bacterial growth curve would produce unexpected results?...
What would be some reasons as to why a bacterial growth curve
would produce unexpected results? For example, an E. coli culture
increases after the stationary phase? Or why there is no
stationary phase? I am trying to
interpret results that I have from a lab experiment.
Viable Cell Concentration vs. Time of Escherichia coli E 1.00E+08 1.00E-07 C 1.00E+06 60 80 100 120 140 0 20 40 Time (minutes) Spectrophotometer -96 Well Plate
To construct a calibration curve we would need to take our stock solution and dilute it...
To construct a calibration curve we would need to take our stock solution and dilute it to different concentrations. We would then test those different concentration in our spectrophotometer to test their absorbance. If we diluted our samples as follows, calculate the resulting concentrations. (HINT: this is dilution which means we can use M1V1=M2V2, where M1 is our original 150 mM concentration, V1 is the volume of the original concentration that we added water to dilute, M2 is the concentration...
this is a bacterial growth curve experiment . please explain
rhe results
Procedure: You will follow the growth of E. coli over the course of the period (3 hrs) by making direct counts of the bacterial suspension by measuring the turbidity of a sample at a given time with a spectrophotometer. The data obtained from the direct counts will be used to plot a partial growth curve. Summary: Turbidity Counts with the Spectrophotometer to measure absorbance at 600. Direct Counts...
3. You are measuring the growth of bacterium using cfu mL·You collect samples at various times and dilute them in 10-fold series (as shown in the 2 row of the table) and plate 0.1 ml of each of these dilutions on five standard lab agar plates. The average number of colonies per plate are below Time of Colonies on plate at each dilution ars 45 290 TNTC TNTC 195 20 TNTC TNTC 200 TNTC too numerous to count ADB MO...
growth rates provide his whole information because they can
tell us something about optimum growth conditions. For example if
you were a plot a series of growth curves at various temperatures
and calculate the growth rates, you could generate a graph similar
to the one at the right.
what is the original growth curve in part 1 obtained at the
optimal temperature for this organism? If yes, explain how you
knew. if not, can you tell at what temperature the...
bacterial growth curve help
whats wrong with each graph using the chart provided.
Table 6.3. Data Used to construct Figure 6.10 and 6.11. Hour Absorbance Cfu/ml 0.16 x 10 0.16 x 10 0.16 x 10 0.16 x 10 0.78 x 10 1.98 x 10 2.53 x 10% 3.13 x 10% 4.38 x 10 5.97× 106 9.41 x 10 11.9 x 10 14.7 x 10 21.0× 106 22.0× 106 22.2 x 10% 0.01 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0...
3. You are measuring the growth of bacterium using cfu mL·You collect samples at various times and dilute them in 10-fold series (as shown in the 2 row of the table) and plate 0.1 ml of each of these dilutions on five standard lab agar plates. The average number of colonies per plate are below Time of Colonies on plate at each dilution ars 45 290 TNTC TNTC 195 20 TNTC TNTC 200 TNTC too numerous to count ADB MO...
What would be some reasons as to why a bacterial growth curve
would produce unexpected results? For example, an E. coli culture
increases after the stationary phase? Or why there is no
stationary phase? I am trying to
interpret results that I have from a lab experiment.
Viable Cell Concentration vs. Time of Escherichia coli E 1.00E+08 1.00E-07 C 1.00E+06 60 80 100 120 140 0 20 40 Time (minutes) Spectrophotometer -96 Well Plate
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