Question

Conditional alleles are mutant gene versions encoding proteins that can function normally at the permissive condition but are defective at the restrictive condition. One commonly used condition is temperature. Conditional alleles are especially useful to geneticists because they permit the study of essential genes. At the permissive temperature, the organism lives normally. When the organism is shifted to the non-permissive temperature, the effect of inactivating the gene can be studied. Which of the alleles shown below (mutations are bold and underlined) is most likely to lead to a conditional allele? Fully explain your choice, indicating why each of the other choices may not lead to a conditional allel. Assume that the coding region of the mRNA is 1000 codons, but only the first few codons having mutation(s) are shown. -AUG GCU UGG AUC GAC- wild-type -AUG GCU UGA AUC GAC- allele 1 -AUG GCU UGG AUA GAC- allele 2 -AUG GCU UGG AUC GAG- allele 3 -AUG GCU UGG AAU CGA C- allele 4 -AUG GCU UGG AAA UCG AC- allele 5
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Answer 1

CONDITIONED ALLELES ARE THE PRODUCT OF EITHER DELETION, INSERTION , INVERSION OR SUBSTITUTION. IF WE SEE ALLELE 1, ALLLELE 2, ALLELE 3 THEY ARE SHOWING NEITHER DELETION, INSERTION OR INVERSION OR SUBSTITUTION ON COMARING WITH WILD TYPE ALLELE. WHERE AS ALLELE 4 AND ALLELE 5 SHOWS INSERTION OF ONE BASE AND 2 BASE RESPECTIVE;Y ;EADING TO FORMTION OF PRODUCTS DIFFERENT FROM WHAT WAS DESIRED.