Ans. Triple sugar iron (TSI) medium consists of three sugars (glucose, lactose and sucrose), sulphur in from of ferrous sulfate, sodium thiosulfate, and phenol red as indicator.
The bacteria which can ferment any of these sugars cause change of the color of the medium. Sulphur reduction by bacteria is exhibited blackening of the medium.
# It is used as differential medium for identification of enteric bacteria including Enterobactor, Salmonella, Shigella, etc.
Background
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Questions
Experiment 2: TSI The triple sugar iron agar (TSI) can identify gram negative bacteria based on how they metabolize carbohydrates. It contains three carbohydrates-glucose, lactose and sucrose, in addition to sodium thiosulfate which some bacteria use in the production of hydrogen sulfide (H2S). It also contains iron in the ferrous form and phenol red as a pH indicator. TSI is poured into a tube when heated to a liquid form, and the tube is slanted as...
Hello please help – How and what specials stains you would use to identify bacteria klebsiella pneumoniae?
You are required to test river water for the presence of bacteria and viruses and to determine their tolerance to heavy metals as well as resistance to antibiotics. Describe in detail various techniques that you would use to achieve these aims.
what do you think the colony shape and colony edge/ margin
are? and also the TSI slant is yellow and butt is red, is it mean
that the TSI is positive to acid?
Membrane fluidity for bacteria is critical. What type of fatty acids would you expect bacteria to synthesize as growth temperature increases? Why type of fatty acids would you expect bacteria to synthesize as growth temperature decreases? Why cannot bacteria simply synthesize the same type of fatty acids regardless of temperature?
What is the media used for lipid hydrolysis test? What is the enzyme bacteria produce for lipid hydrolysis? li pases What are the products of lipid hydrolysis? The process that convert fatty acids to acetyl CoA is called What would you observe in a positive test for lipid hydrolysis?
What is hemolysis? How do you test the hemolytic activity of certain bacteria? What kind of hemolytic activity do you know?
give a background and explanation for each of the following microbial tests Oxidase test, TSI (KIA) test, The Enterotube II® System
TSI Triple Sugar Iron Agar Data Sheet The enterics, specifically coliforms, will show what result in the TSI? What is reversion and what organisms showed reversion in the TSI? What does a black color in the butt of the slant indicate in the TSI? What is the pH indicator in the TSI and what is the purpose of the pH indicator? Do all organisms use the sugars in the TSI? What organisms do not utilize the sugars and what is...
What kind of test would you use? Test the hypothesis that the marital status of individuals is equally distributed among four groups.